Detailed information of TA system TA230943

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 534345..534950
• Replicon: chromosome
• Accession: NZ_CP090508
• Organism: Xylella fastidiosa subsp. morus strain Riv11

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B2I5N3
• Locus tag: LZ758_RS02550
• Protein ID: WP_004089252.1
• Coordinates: 534654..534950 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LZ758_RS02545
• Protein ID: WP_038211623.1
• Coordinates: 534345..534650 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ758_RS02505 (LZ758_02505)	530734..531042	-	309	WP_004085003.1	putative addiction module antidote protein	-
LZ758_RS02510 (LZ758_02510)	531046..531345	-	300	WP_024749166.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LZ758_RS02515 (LZ758_02515)	531433..531738	+	306	WP_230577600.1	hypothetical protein	-
LZ758_RS02520 (LZ758_02520)	531882..532202	+	321	WP_234509108.1	hypothetical protein	-
LZ758_RS02525 (LZ758_02525)	532202..532519	+	318	WP_012382585.1	hypothetical protein	-
LZ758_RS02530 (LZ758_02530)	532516..533346	+	831	WP_234056377.1	hypothetical protein	-
LZ758_RS02535 (LZ758_02535)	533343..533984	+	642	WP_040123250.1	Gp138 family membrane-puncturing spike protein	-
LZ758_RS02540 (LZ758_02540)	533981..534334	+	354	WP_234056392.1	hypothetical protein	-
LZ758_RS02545 (LZ758_02545)	534345..534650	-	306	WP_038211623.1	putative addiction module antidote protein	Antitoxin
LZ758_RS02550 (LZ758_02550)	534654..534950	-	297	WP_004089252.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LZ758_RS02555 (LZ758_02555)	535049..536194	+	1146	WP_038211620.1	baseplate J/gp47 family protein	-
LZ758_RS02560 (LZ758_02560)	536191..536751	+	561	WP_011097869.1	DUF2612 domain-containing protein	-
LZ758_RS02565 (LZ758_02565)	536755..537918	+	1164	WP_040123248.1	tail fiber protein	-
LZ758_RS02570 (LZ758_02570)	538215..538367	+	153	WP_162531331.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	433970..537918	103948

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (50-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10790.57 Da
• Isoelectric Point: 10.0509

>T230943 WP_004089252.1 NZ_CP090508:c534950-534654 [Xylella fastidiosa subsp. morus]
MVELIKTSTFDAWINSLRDRKAAARIQARLDRLALGNPGDVKPVGAGISEMRIDHGPGYRIYFMKHGAVLILLLCGGDKS
SQVRDIEQAKALAALWKD

Nucleotide

• Length: 297 bp

>T230943 NZ_CP125731:2404567-2405889 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGCCTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10982.42 Da
• Isoelectric Point: 5.0941

>AT230943 WP_038211623.1 NZ_CP090508:c534650-534345 [Xylella fastidiosa subsp. morus]
MNNETFSRYDTADYLKTEEDIAAYMEAVMEEGGRDNPAFIARALGAVARARNLSQLARDVGMSRQGLDKALSIDGNPSFS
TILKVAKALGLRMSFTPSSMS

Nucleotide

• Length: 306 bp

>AT230943 NZ_CP125731:2404301-2404567 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A060HBK9