Detailed information of TA system TA230940

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 483403..483995
• Replicon: chromosome
• Accession: NZ_CP090508
• Organism: Xylella fastidiosa subsp. morus strain Riv11

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A060HCK8
• Locus tag: LZ758_RS02170
• Protein ID: WP_004085590.1
• Coordinates: 483699..483995 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: LZ758_RS02165
• Protein ID: WP_004085592.1
• Coordinates: 483403..483696 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ758_RS02125 (LZ758_02125)	479746..480054	-	309	WP_004085003.1	putative addiction module antidote protein	-
LZ758_RS02130 (LZ758_02130)	480058..480357	-	300	WP_024749166.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LZ758_RS02135 (LZ758_02135)	480445..480750	+	306	WP_230577600.1	hypothetical protein	-
LZ758_RS02140 (LZ758_02140)	480766..481215	+	450	WP_230577599.1	hypothetical protein	-
LZ758_RS02145 (LZ758_02145)	481215..481532	+	318	WP_012382585.1	hypothetical protein	-
LZ758_RS02150 (LZ758_02150)	481529..482359	+	831	WP_234056377.1	hypothetical protein	-
LZ758_RS02155 (LZ758_02155)	482356..482997	+	642	WP_040123250.1	Gp138 family membrane-puncturing spike protein	-
LZ758_RS02160 (LZ758_02160)	482994..483347	+	354	WP_040123277.1	hypothetical protein	-
LZ758_RS02165 (LZ758_02165)	483403..483696	-	294	WP_004085592.1	putative addiction module antidote protein	Antitoxin
LZ758_RS02170 (LZ758_02170)	483699..483995	-	297	WP_004085590.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LZ758_RS02175 (LZ758_02175)	484098..485243	+	1146	WP_038211620.1	baseplate J/gp47 family protein	-
LZ758_RS02180 (LZ758_02180)	485240..485800	+	561	WP_234056378.1	DUF2612 domain-containing protein	-
LZ758_RS02185 (LZ758_02185)	485804..486967	+	1164	WP_234056379.1	tail fiber protein	-
LZ758_RS02190 (LZ758_02190)	487214..487867	+	654	WP_234056380.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	433970..537918	103948

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Uncharacterized protein HI1420 (3-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11169.70 Da
• Isoelectric Point: 10.0610

>T230940 WP_004085590.1 NZ_CP090508:c483995-483699 [Xylella fastidiosa subsp. morus]
MIELKQTDTFRKWREKLKDARARSAIASRLDRLAFGHVGDAEPVGKGVSELRINYGPGYRVYFQRRGDTIYLLLCGGDKG
SQARDIKTALHLSEQWSE

Nucleotide

• Length: 297 bp

>T230940 NZ_CP125731:1881057-1881308 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10246.59 Da
• Isoelectric Point: 5.6232

>AT230940 WP_004085592.1 NZ_CP090508:c483696-483403 [Xylella fastidiosa subsp. morus]
MTEKLTSYDPAEDLTTDQAIADFMAAAFGTNDPAYVAHALGVVARAKGMTQIASQTGLSREQLYRSFSAEGNPTLRTTLA
VMKALGIELSAKPSGVH

Nucleotide

• Length: 294 bp

>AT230940 NZ_CP125731:1881305-1881559 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A060HCK8