Detailed information of TA system TA023093

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1799386..1799976
• Replicon: chromosome
• Accession: NC_011283
• Organism: Klebsiella pneumoniae 342

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: KPK_RS08380
• Protein ID: WP_012541133.1
• Coordinates: 1799644..1799976 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A1F2LZQ3
• Locus tag: KPK_RS08375
• Protein ID: WP_012541132.1
• Coordinates: 1799386..1799643 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPK_RS08355	1794995..1795570	+	576	WP_012541129.1	hypothetical protein	-
KPK_RS08360	1795749..1796585	+	837	WP_008804155.1	alpha/beta hydrolase	-
KPK_RS08365	1796791..1797762	+	972	WP_012541130.1	sensor domain-containing diguanylate cyclase	-
KPK_RS08370	1797759..1798859	-	1101	WP_012541131.1	phosphotransferase	-
KPK_RS08375	1799386..1799643	+	258	WP_012541132.1	antitoxin	Antitoxin
KPK_RS08380	1799644..1799976	+	333	WP_012541133.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KPK_RS08390	1800299..1801735	+	1437	WP_041165335.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
KPK_RS08400	1802108..1803562	-	1455	WP_008804170.1	AMP nucleosidase	-
KPK_RS08405	1803693..1803938	-	246	WP_012541134.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11819.67 Da
• Isoelectric Point: 10.1839

>T23093 WP_012541133.1 NC_011283:1799644-1799976 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNVARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Nucleotide

• Length: 333 bp

>T23093 NC_011283:1799644-1799976 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTCGTGGTTCCCGTCACCAGCGGTGGAAACGTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCTGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTCAGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9420.91 Da
• Isoelectric Point: 4.4915

>AT23093 WP_012541132.1 NC_011283:1799386-1799643 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT23093 NC_011283:1799386-1799643 [Klebsiella variicola]
ATGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAGCGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1F2LZQ3