Detailed information of TA system TA230915

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 1491790..1492372
• Replicon: chromosome
• Accession: NZ_CP090504
• Organism: Xylella fastidiosa subsp. morus strain Riv25

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A060HA48
• Locus tag: LZ755_RS07100
• Protein ID: WP_038229226.1
• Coordinates: 1492082..1492372 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A060HE88
• Locus tag: LZ755_RS07095
• Protein ID: WP_004091409.1
• Coordinates: 1491790..1492095 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ755_RS07060 (LZ755_07060)	1487045..1488043	+	999	WP_004085391.1	MBL fold metallo-hydrolase	-
LZ755_RS07065 (LZ755_07065)	1488009..1489203	+	1195	Protein_1380	DUF5710 domain-containing protein	-
LZ755_RS07070 (LZ755_07070)	1489327..1489752	+	426	WP_038229235.1	antirestriction protein	-
LZ755_RS07075 (LZ755_07075)	1490020..1490277	+	258	WP_038229233.1	hypothetical protein	-
LZ755_RS07080 (LZ755_07080)	1490362..1490928	-	567	WP_038229232.1	recombinase family protein	-
LZ755_RS07085 (LZ755_07085)	1491016..1491303	-	288	WP_038229230.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LZ755_RS07090 (LZ755_07090)	1491296..1491538	-	243	WP_004085758.1	type II toxin-antitoxin system ParD family antitoxin	-
LZ755_RS07095 (LZ755_07095)	1491790..1492095	-	306	WP_004091409.1	BrnA antitoxin family protein	Antitoxin
LZ755_RS07100 (LZ755_07100)	1492082..1492372	-	291	WP_038229226.1	BrnT family toxin	Toxin
LZ755_RS12265	1492447..1492722	+	276	WP_004091404.1	XRE family transcriptional regulator	-
LZ755_RS07110 (LZ755_07110)	1492833..1493153	+	321	WP_004091397.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LZ755_RS07115 (LZ755_07115)	1493283..1493609	+	327	WP_004091396.1	DNA-binding protein	-
LZ755_RS07120 (LZ755_07120)	1493751..1494677	-	927	WP_051606204.1	hypothetical protein	-
LZ755_RS07125 (LZ755_07125)	1495083..1495499	-	417	WP_038229223.1	IS200/IS605 family transposase	-
LZ755_RS07130 (LZ755_07130)	1495522..1496679	+	1158	WP_038229247.1	RNA-guided endonuclease TnpB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1488745..1510945	22200
	-	inside	IScluster/Tn	-	-	1490362..1496679	6317

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-89)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (11-93)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11304.62 Da
• Isoelectric Point: 6.0917

>T230915 WP_038229226.1 NZ_CP090504:c1492372-1492082 [Xylella fastidiosa subsp. morus]
VERYFEWDDKKAESNFRKHGIRFDEAALAFDDPFAVSEQNRIENGEQRWQTIGMAGGCLLLLVAHTVHFEDDGIEVVRII
SARRVDRKERRRYEHG

Nucleotide

• Length: 291 bp

>T230915 NZ_CP125660:2069493-2069585 [Bacillus subtilis]
ATGTCAACATTCCAGGCATTAATGCTAATGCTTGCAATCGGGTCGTTTATAATCGCCCTATTGACGTATATAGAAAAAAT
AGACCTCCCTTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11684.43 Da
• Isoelectric Point: 9.4870

>AT230915 WP_004091409.1 NZ_CP090504:c1492095-1491790 [Xylella fastidiosa subsp. morus]
MNMVRYKQSELPPLTEERKAELKALAKKPDSEIDYSDIPPLDESFWARAVPNPFFKPVKTHASVRIDSDVLAWLKSQGKG
YQTRMNAILREAMLRSLHQQE

Nucleotide

• Length: 306 bp

>AT230915 NZ_CP125660:c2069739-2069566 [Bacillus subtilis]
TTACAATACATTACAGTGCATTTCTTTAATGAGAAATGCATAAAATAAAAAAGACCGAGGTGCTACCAACACCCCGGCTC
TGTACAAAAAGTTGCCCATCAAAGGGCTTGCTCGATGTGGTTCTAGGTAGGCCTATCCCTTCAGGTTCTGAGGCTCAAGG
GAGGTCTATTTTTT

Structures

Toxin

• AlphaFold DB: A0A060HA48

Antitoxin

• AlphaFold DB: A0A060HE88