Detailed information of TA system TA230912

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 970239..970825
• Replicon: chromosome
• Accession: NZ_CP090504
• Organism: Xylella fastidiosa subsp. morus strain Riv25

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LZ755_RS04500
• Protein ID: WP_038228269.1
• Coordinates: 970239..970493 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: B2I6C9
• Locus tag: LZ755_RS04505
• Protein ID: WP_004085025.1
• Coordinates: 970490..970825 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ755_RS04465 (LZ755_04465)	965946..966551	-	606	WP_040123201.1	conjugal transfer protein TrbN	-
LZ755_RS04470 (LZ755_04470)	966557..966931	-	375	WP_040123200.1	hypothetical protein	-
LZ755_RS04475 (LZ755_04475)	967078..967332	+	255	WP_040123199.1	hypothetical protein	-
LZ755_RS04480 (LZ755_04480)	967329..968231	+	903	WP_234056418.1	YdaU family protein	-
LZ755_RS04485 (LZ755_04485)	968212..968901	+	690	WP_038228265.1	hypothetical protein	-
LZ755_RS04490 (LZ755_04490)	968898..969359	+	462	WP_234056374.1	hypothetical protein	-
LZ755_RS04495 (LZ755_04495)	969488..970186	+	699	WP_038228267.1	hypothetical protein	-
LZ755_RS04500 (LZ755_04500)	970239..970493	+	255	WP_038228269.1	type II toxin-antitoxin system HicA family toxin	Toxin
LZ755_RS04505 (LZ755_04505)	970490..970825	+	336	WP_004085025.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LZ755_RS04510 (LZ755_04510)	971005..971304	-	300	WP_004089519.1	HigA family addiction module antitoxin	-
LZ755_RS04515 (LZ755_04515)	971314..971595	-	282	WP_011098058.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LZ755_RS04520 (LZ755_04520)	971943..972657	+	715	Protein_884	site-specific DNA-methyltransferase	-
LZ755_RS04525 (LZ755_04525)	972644..973108	-	465	WP_011098056.1	low molecular weight protein-tyrosine-phosphatase	-
LZ755_RS04530 (LZ755_04530)	973105..973878	-	774	WP_004089528.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
LZ755_RS04535 (LZ755_04535)	974313..975224	+	912	WP_230577717.1	iron-sulfur cluster carrier protein ApbC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-80)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9679.16 Da
• Isoelectric Point: 11.2766

>T230912 WP_038228269.1 NZ_CP090504:970239-970493 [Xylella fastidiosa subsp. morus]
MKRKHAKTLSAIYTRPVSANIQWRDIEALFVELGAKVEEREGSRMLVRLFGERRIFHRPHPSHNTDKGAVESIRAWLKSN
GVTP

Nucleotide

• Length: 255 bp

>T230912 NZ_CP125658:1113577-1113843 [Enterococcus sp. ZQ21]
ATGACCTACCGTTTAGAATTCACGCCTGAAATTCAGAAACAACTTCGTAAAATGGACAAGCATCAAGCGACGCTGATCGT
TCGATGGCTATATCAACATATTGATGGTATTGACGATCCGAGAAAATTCGGAAAAGGATTGACCGCTAATCGTTCAGGAC
AATGGCGTTATCGAATTGGCAAATATCGTGTGTTGGTAGAGATAGAAGATAATCAAATAGTTGTTATCGCTATTCAAGTG
GGACACCGAAAAAATGTCTATGATTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12324.07 Da
• Isoelectric Point: 5.6556

>AT230912 WP_004085025.1 NZ_CP090504:970490-970825 [Xylella fastidiosa subsp. morus]
MKNIMTIESFKAIITYDPEIDMFRGEFVGINGGADFYAKDLKGLRREGAISLKVFLEACKEDSVEPRKCYSGKFNARISP
ELHALASEAAAAQGISLNQFVEQAIQHEVHT

Nucleotide

• Length: 336 bp

>AT230912 NZ_CP125658:1113350-1113580 [Enterococcus sp. ZQ21]
ATGGCTATGATTACGGTTCGTGTGAGTGATGCCGAAAAAGAATGGCTTTCTTATATGGCGGAATTTTATGGGATTACTTT
ATCTGAGCTTCTAAAAACCTATTCGATGGAACAACTAGAAGATGAATACGATCGTCAAACGGCTGAAATTGCCCATAAAC
ATTGGGTAGAAAGTGGAAAAACAACAGTATCCATGGAAGAAATCCTTCGTGAATTTGGAGGGTTGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A060HAK5