Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/ElaA-DUF1778
Location 178827..179578 Replicon plasmid pKP187
Accession NC_011282
Organism Klebsiella pneumoniae 342

Toxin (Protein)


Gene name ataT Uniprot ID A0A486R6L7
Locus tag KPK_RS27360 Protein ID WP_041165502.1
Coordinates 178827..179309 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A6S4YWG5
Locus tag KPK_RS27365 Protein ID WP_012540170.1
Coordinates 179300..179578 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPK_RS27345 175936..176781 + 846 Protein_173 IS30 family transposase -
KPK_RS29810 176954..177151 - 198 WP_077251736.1 bacteriocin immunity protein -
KPK_RS27350 177215..178048 - 834 WP_012540133.1 GIY-YIG nuclease family protein -
KPK_RS27355 178385..178789 - 405 WP_004210282.1 DUF2251 domain-containing protein -
KPK_RS27360 178827..179309 - 483 WP_041165502.1 GNAT family N-acetyltransferase Toxin
KPK_RS27365 179300..179578 - 279 WP_012540170.1 DUF1778 domain-containing protein Antitoxin
KPK_RS27370 179886..180422 + 537 WP_012540072.1 hypothetical protein -
KPK_RS27375 181427..181741 + 315 WP_012540228.1 hypothetical protein -
KPK_RS27380 181985..182467 - 483 WP_004210306.1 hypothetical protein -
KPK_RS27385 182939..183874 - 936 WP_004026564.1 omptin family outer membrane protease Kop -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..187922 187922


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(44-144)

Antitoxin

Vibrio phage ICP1, Orf50

(6-92)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17697.61 Da        Isoelectric Point: 9.4948

>T23090 WP_041165502.1 NC_011282:c179309-178827 [Klebsiella variicola]
MGMRPPEPLTPEHNIADFCCQDQVLSEWLKKKALKNHSTGLSRVFVVCAENTNRVIAYYCLASGSVHRNTVPGAYRRNAP
EALPVIVLGRLAVDAAWARKGLGAALLKDAIYRTQHIAIQVGVRALLVHALNDEVREFYTKFGFEPSIANALTLLFPIKT

Download         Length: 483 bp

>T23090 NC_011282:c179309-178827 [Klebsiella variicola]
ATGGGAATGAGGCCTCCGGAACCTCTTACCCCTGAACACAACATTGCTGATTTTTGTTGTCAGGATCAGGTTCTTTCTGA
GTGGTTGAAGAAAAAGGCGCTGAAGAACCATAGCACGGGCCTTTCAAGAGTTTTCGTCGTCTGTGCTGAAAATACGAATA
GGGTAATTGCGTACTACTGTTTGGCATCCGGAAGCGTGCACAGAAACACCGTACCCGGAGCATACCGTCGTAATGCACCA
GAGGCTTTACCGGTCATTGTTCTGGGAAGGCTTGCGGTTGACGCTGCCTGGGCAAGAAAAGGGCTGGGAGCTGCACTGCT
TAAGGATGCGATATACCGAACACAACATATTGCAATACAGGTTGGTGTTAGGGCGCTTCTTGTTCACGCCTTAAATGATG
AAGTTAGGGAATTTTACACAAAGTTTGGATTCGAGCCCTCCATTGCCAATGCGCTGACATTATTATTTCCGATCAAAACT
TGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10749.33 Da        Isoelectric Point: 4.6722

>AT23090 WP_012540170.1 NC_011282:c179578-179300 [Klebsiella variicola]
MKPDIREAPINIRAKTSQRDLIDMAANLVSKSRTDFMLEVACREAQDILLDQRLFLLDDKQFNAFVEELDAPMTPERQAR
IDNLMNRKSPWE

Download         Length: 279 bp

>AT23090 NC_011282:c179578-179300 [Klebsiella variicola]
ATGAAACCAGACATTCGTGAGGCTCCGATAAACATCCGGGCGAAAACATCTCAACGCGATCTTATCGACATGGCCGCAAA
TCTTGTATCTAAATCACGTACTGATTTTATGCTGGAAGTGGCCTGCCGCGAAGCGCAGGACATCTTGTTAGATCAGCGCT
TGTTTCTGCTGGATGATAAGCAGTTTAACGCGTTTGTCGAAGAGCTTGACGCCCCTATGACCCCAGAGCGTCAGGCGCGT
ATTGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486R6L7

Antitoxin

Source ID Structure
AlphaFold DB A0A6S4YWG5

References