Detailed information of TA system TA023089

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 119339..119982
• Replicon: plasmid pKP187
• Accession: NC_011282
• Organism: Klebsiella pneumoniae 342

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: KPK_RS27055
• Protein ID: WP_012540249.1
• Coordinates: 119339..119755 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: KPK_RS27060
• Protein ID: WP_001261276.1
• Coordinates: 119752..119982 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPK_RS27040	114389..116497	-	2109	WP_012540154.1	peptidase domain-containing ABC transporter	-
KPK_RS27045	116487..117764	-	1278	WP_012540144.1	HlyD family secretion protein	-
KPK_RS27050	118420..119298	+	879	WP_012540096.1	DUF3644 domain-containing protein	-
KPK_RS27055	119339..119755	-	417	WP_012540249.1	type II toxin-antitoxin system VapC family toxin	Toxin
KPK_RS27060	119752..119982	-	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KPK_RS29765	120114..120455	+	342	Protein_115	IS3 family transposase	-
KPK_RS27070	120461..120715	-	255	WP_012540245.1	hypothetical protein	-
KPK_RS27075	121603..123813	-	2211	WP_012540226.1	AAA family ATPase	-
KPK_RS27080	124180..124680	+	501	WP_012540042.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..187922	187922
	-	flank	IS/Tn	-	-	120069..120455	386

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15050.53 Da
• Isoelectric Point: 8.5403

>T23089 WP_012540249.1 NC_011282:c119755-119339 [Klebsiella variicola]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T23089 NC_011282:c119755-119339 [Klebsiella variicola]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGACCGGATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCTGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATAGC
CGGCCACGCCATCGCGGCCGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT23089 WP_001261276.1 NC_011282:c119982-119752 [Klebsiella variicola]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT23089 NC_011282:c119982-119752 [Klebsiella variicola]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTCCCCCGTGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGAGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCATTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGTGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A387K023