Detailed information of TA system TA230884

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 492281..492918
• Replicon: chromosome
• Accession: NZ_CP090477
• Organism: Bacillus amyloliquefaciens strain W0101

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: LXM91_RS02530
• Protein ID: WP_003156187.1
• Coordinates: 492568..492918 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: LXM91_RS02525
• Protein ID: WP_003156188.1
• Coordinates: 492281..492562 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXM91_RS02505 (LXM91_02505)	488646..489245	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
LXM91_RS02510 (LXM91_02510)	489338..489703	+	366	WP_007410229.1	holo-ACP synthase	-
LXM91_RS02515 (LXM91_02515)	489868..490875	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
LXM91_RS02520 (LXM91_02520)	490992..492161	+	1170	WP_039252648.1	alanine racemase	-
LXM91_RS02525 (LXM91_02525)	492281..492562	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
LXM91_RS02530 (LXM91_02530)	492568..492918	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
LXM91_RS02535 (LXM91_02535)	493036..493857	+	822	WP_003156182.1	STAS domain-containing protein	-
LXM91_RS02540 (LXM91_02540)	493862..494227	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
LXM91_RS02545 (LXM91_02545)	494230..494631	+	402	WP_003156178.1	anti-sigma regulatory factor	-
LXM91_RS02550 (LXM91_02550)	494643..495650	+	1008	WP_039252645.1	PP2C family protein-serine/threonine phosphatase	-
LXM91_RS02555 (LXM91_02555)	495714..496043	+	330	WP_031378636.1	anti-sigma factor antagonist	-
LXM91_RS02560 (LXM91_02560)	496040..496522	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
LXM91_RS02565 (LXM91_02565)	496488..497276	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
LXM91_RS02570 (LXM91_02570)	497276..497878	+	603	WP_039252641.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T230884 WP_003156187.1 NZ_CP090477:492568-492918 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T230884 NZ_CP125637:c495961-495578 [Rhizobium leguminosarum]
GTGATACTGGCAGACACTTCTATATGGATCGATCATTTCCGCCATGTTGATGCGGAACTTCGGACGATCATTGAGGACGA
TCGTCTACTCTGCCATCCGGCTGTAGTTGGCGAACTGGCGCTTGGCAGCCTTCGAGATCGCGGGCGCGTGATAGCGTTTC
TGGCAGCCCAGCGCCAAGCGTTCGTCGCAACGCATGACGAAGTGATGACAATGATTGATCGTCACGGCATTTTCAGTATG
GGCATCGGCTATACAGATGCCCACTTGCTCGCCTCGATACTGCTTGATCGGCGAGCGGCGTTATGGACCAGGGACAAGCG
TCTTCAGGCGGCGGCCAAAAAGGCAGGCGCTTCATTGCATAAGCCGGCCAATGCTCGCAACTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT230884 WP_003156188.1 NZ_CP090477:492281-492562 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT230884 NZ_CP125637:c496158-495958 [Rhizobium leguminosarum]
ATGCGATCGACTATCAATCTAGACGACACTCTCATGGAAAGGGCCAGGTCCCTGACCGGCACAAAAGAAACCGCTGCTAT
AGTCCGCCAGGCGTTGGAGACGCTTGTTCGAGTAGAGTCCGGAAAACGCCTCATCGCGCTCGGCGGAACTATGCCCGATG
CAGAGGCAGCTCCACGTCGCCGGAGCGCAGCTGCCAAGTGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5