Detailed information of TA system TA023088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 108700..109436
• Replicon: plasmid pKP187
• Accession: NC_011282
• Organism: Klebsiella pneumoniae 342

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: KPK_RS26985
• Protein ID: WP_012540079.1
• Coordinates: 108700..109182 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: KPK_RS26990
• Protein ID: WP_012540130.1
• Coordinates: 109170..109436 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPK_RS29750	105180..105539	+	360	Protein_96	hypothetical protein	-
KPK_RS29755	105848..106441	-	594	WP_101825997.1	helix-turn-helix domain-containing protein	-
KPK_RS26975	106626..107045	-	420	WP_012540063.1	ASCH domain-containing protein	-
KPK_RS26980	107233..108482	-	1250	Protein_99	ISNCY family transposase	-
KPK_RS26985	108700..109182	-	483	WP_012540079.1	GNAT family N-acetyltransferase	Toxin
KPK_RS26990	109170..109436	-	267	WP_012540130.1	DUF1778 domain-containing protein	Antitoxin
KPK_RS26995	109630..109860	-	231	WP_012540060.1	hypothetical protein	-
KPK_RS27000	109874..110077	-	204	WP_012540085.1	hemolysin expression modulator Hha	-
KPK_RS27005	110110..110469	-	360	WP_012540187.1	hypothetical protein	-
KPK_RS27010	110514..110759	-	246	Protein_105	DNA-binding protein	-
KPK_RS27020	111314..111895	+	582	WP_041165492.1	hypothetical protein	-
KPK_RS27025	112034..112981	-	948	Protein_107	Abi family protein	-
KPK_RS29760	113302..113646	+	345	WP_012540121.1	hypothetical protein	-
KPK_RS27035	113754..114389	-	636	WP_009309912.1	CPBP family intramembrane metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..187922	187922
	-	flank	IS/Tn	-	-	107233..108285	1052

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17398.09 Da
• Isoelectric Point: 9.5822

>T23088 WP_012540079.1 NC_011282:c109182-108700 [Klebsiella variicola]
VGRITAPEPLSSAHQLAEFVSGEMVLDEWLKQRSLKNQALGAARTFVVCKTGTKQVTGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T23088 NC_011282:c109182-108700 [Klebsiella variicola]
GTGGGACGTATAACTGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGGGAAATGGTACTCGA
TGAATGGTTAAAACAGAGAAGTTTAAAAAATCAGGCCCTTGGCGCTGCACGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAACTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATTCTCGCTCGCCTAGCGGTAGATGTTTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10162.65 Da
• Isoelectric Point: 4.6558

>AT23088 WP_012540130.1 NC_011282:c109436-109170 [Klebsiella variicola]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLNAPVEDELAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT23088 NC_011282:c109436-109170 [Klebsiella variicola]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTCATTCTTGAGATGGCTTGCAAGGCTGCAGAGAACGTGATCCTTGATCGTCGTGTTTTCAATT
TTAACGACGAACAGTATGCTGAGTTCATCGATATGCTCAATGCACCAGTCGAGGATGAGCTTGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA