Detailed information of TA system TA230876

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 91777..92447
• Replicon: plasmid p1
• Accession: NZ_CP090469
• Organism: Klebsiella pneumoniae strain kp7450

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: LZ687_RS26135
• Protein ID: WP_004213072.1
• Coordinates: 91777..92220 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: LZ687_RS26140
• Protein ID: WP_004213073.1
• Coordinates: 92217..92447 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ687_RS26100 (LZ687_26100)	87186..87461	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
LZ687_RS26105 (LZ687_26105)	87524..88015	+	492	WP_011251327.1	DM13 domain-containing protein	-
LZ687_RS26110 (LZ687_26110)	88064..88984	+	921	WP_029884592.1	DUF1471 domain-containing protein	-
LZ687_RS26115 (LZ687_26115)	89075..89478	+	404	Protein_97	GAF domain-containing protein	-
LZ687_RS26120 (LZ687_26120)	89996..90634	-	639	WP_032488580.1	mucoid phenotype regulator RmpA2	-
LZ687_RS26125 (LZ687_26125)	91050..91354	+	305	Protein_99	transposase	-
LZ687_RS26130 (LZ687_26130)	91377..91628	-	252	WP_186987481.1	hypothetical protein	-
LZ687_RS26135 (LZ687_26135)	91777..92220	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
LZ687_RS26140 (LZ687_26140)	92217..92447	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LZ687_RS26145 (LZ687_26145)	93055..94188	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
LZ687_RS26150 (LZ687_26150)	94204..94497	+	294	WP_004213076.1	hypothetical protein	-
LZ687_RS26155 (LZ687_26155)	94487..94693	-	207	WP_004213077.1	hypothetical protein	-
LZ687_RS26160 (LZ687_26160)	95045..95335	+	291	WP_004213078.1	hypothetical protein	-
LZ687_RS26165 (LZ687_26165)	95325..96224	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iucA / iucB / iucC / iucD / iutA / rmpA / iutA / iutA / rmpA	1..165596	165596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T230876 WP_004213072.1 NZ_CP090469:c92220-91777 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T230876 NZ_CP125620:c3119894-3119502 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT230876 WP_004213073.1 NZ_CP090469:c92447-92217 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT230876 NZ_CP125620:c3120106-3119891 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620