Detailed information of TA system TA230869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2748978..2750566
• Replicon: chromosome
• Accession: NZ_CP090468
• Organism: Klebsiella pneumoniae strain kp7450

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LZ687_RS13290
• Protein ID: WP_004179815.1
• Coordinates: 2748978..2750300 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LZ687_RS13295
• Protein ID: WP_004176198.1
• Coordinates: 2750300..2750566 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ687_RS13275 (2744034)	2744034..2746535	+	2502	WP_020323802.1	fimbria/pilus outer membrane usher protein	-
LZ687_RS13280 (2746523)	2746523..2747527	+	1005	WP_004179822.1	fimbrial protein	-
LZ687_RS13285 (2747669)	2747669..2748961	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
LZ687_RS13290 (2748978)	2748978..2750300	-	1323	WP_004179815.1	type II toxin-antitoxin system HipA family toxin	Toxin
LZ687_RS13295 (2750300)	2750300..2750566	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LZ687_RS13300 (2750832)	2750832..2751158	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
LZ687_RS13305 (2751155)	2751155..2751655	-	501	WP_004179811.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LZ687_RS13310 (2751633)	2751633..2753012	-	1380	WP_004179808.1	nucleobase:cation symporter-2 family protein	-
LZ687_RS13315 (2753358)	2753358..2754512	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
LZ687_RS13320 (2754493)	2754493..2755416	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	2541189..2785401	244212

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49152.42 Da
• Isoelectric Point: 7.2850

>T230869 WP_004179815.1 NZ_CP090468:c2750300-2748978 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTHQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T230869 NZ_CP125620:c2854919-2854524 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT230869 WP_004176198.1 NZ_CP090468:c2750566-2750300 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT230869 NZ_CP125620:c2855161-2854916 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA