Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 29722..30632 Replicon plasmid pKP187
Accession NC_011282
Organism Klebsiella pneumoniae 342

Toxin (Protein)


Gene name - Uniprot ID -
Locus tag KPK_RS26645 Protein ID WP_012540044.1
Coordinates 30162..30632 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag KPK_RS26640 Protein ID WP_004026357.1
Coordinates 29722..30165 (+) Length 148 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPK_RS26615 25274..26332 + 1059 WP_012540183.1 sensor domain-containing diguanylate cyclase -
KPK_RS26620 26687..27379 + 693 WP_012540197.1 EAL domain-containing protein -
KPK_RS26635 28580..29622 + 1043 Protein_27 translesion error-prone DNA polymerase V subunit UmuC -
KPK_RS26640 29722..30165 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
KPK_RS26645 30162..30632 + 471 WP_012540044.1 RES family NAD+ phosphorylase Toxin
KPK_RS26650 30743..31003 - 261 WP_041165481.1 hypothetical protein -
KPK_RS30305 31702..33071 + 1370 WP_123809109.1 IS3 family transposase -
KPK_RS26665 33289..33639 + 351 WP_012540252.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
KPK_RS26670 33687..34049 + 363 WP_012540111.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..187922 187922
- flank IS/Tn - - 32268..33071 803


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-148)

Antitoxin

(30-89)


Sequences


Toxin        


Download         Length: 157 a.a.        Molecular weight: 17545.82 Da        Isoelectric Point: 4.6155

>T23086 WP_012540044.1 NC_011282:30162-30632 [Klebsiella variicola]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDLPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSYTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T23086 NC_011282:30162-30632 [Klebsiella variicola]
GTGATCCTCTACAGGCTGACGAAAACAAAATATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATCTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGCGATGCCTGGTCATATACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT23086 WP_004026357.1 NC_011282:29722-30165 [Klebsiella variicola]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT23086 NC_011282:29722-30165 [Klebsiella variicola]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTTTGGGCCAAATCAATGAAGGTCTGGACGGGAAGGTGGCGAACCGGATAACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCTCGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References