Detailed information of TA system TA230857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1228..1702
• Replicon: plasmid p5
• Accession: NZ_CP090467
• Organism: Klebsiella pneumoniae strain kp5152

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: LZ688_RS29430
• Protein ID: WP_004197762.1
• Coordinates: 1228..1494 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: LZ688_RS29435
• Protein ID: WP_000879771.1
• Coordinates: 1478..1702 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ688_RS29420 (LZ688_29420)	197..742	+	546	WP_023317533.1	hypothetical protein	-
LZ688_RS29425 (LZ688_29425)	808..1158	+	351	WP_004197756.1	hypothetical protein	-
LZ688_RS29430 (LZ688_29430)	1228..1494	-	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LZ688_RS29435 (LZ688_29435)	1478..1702	-	225	WP_000879771.1	DUF6290 family protein	Antitoxin
LZ688_RS29440 (LZ688_29440)	2369..2581	-	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
LZ688_RS29445 (LZ688_29445)	2588..3097	-	510	WP_032433972.1	MbeB family mobilization protein	-
LZ688_RS29450 (LZ688_29450)	3132..3566	-	435	Protein_7	relaxase/mobilization nuclease domain-containing protein	-
LZ688_RS29455 (LZ688_29455)	3775..4119	-	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
LZ688_RS29460 (LZ688_29460)	4109..4285	-	177	WP_004197757.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T230857 WP_004197762.1 NZ_CP090467:c1494-1228 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T230857 NZ_CP125620:c2183750-2183454 [Mycobacterium tuberculosis]
GTGAGTAGCCGATACCTTCTCTCGCCTGCCGCGCAGGCACATCTGGAAGAGATCTGGGACTGCACCTATGACCGTTGGGG
TGTCGATCAGGCCGAGCAGTACCTGCGCGAACTTCAACACGCTATCGACCGTGCTGCGGCAAACCCGCGGATCGGACGAG
CGTGCGACGAGATTCGCCCCGGCTATCGCAAGCTCTCGGCCGGGTCACACACGTTGTTCTATCGGGTGACTGGCGAAGGC
ACCATCGACGTCGTGCGAGTCCTGCACCAACGGATGGACGTCGACCGGAACCTCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT230857 WP_000879771.1 NZ_CP090467:c1702-1478 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT230857 NZ_CP125620:c2183998-2183747 [Mycobacterium tuberculosis]
GTGGGTAAGAACACGTCCTTCGTCCTCGACGAGCACTACAGCGCCTTCATCGACGGCGAGATCGCCGCGGGCCGCTACCG
GTCGGCCAGTGAAGTCATCCGCTCCGCGTTGCGACTGCTCGAGGACCGTGAAACCCAGCTGCGCGCGCTTCGTGAGGCTC
TCGAGGCCGGCGAACGCAGCGGCAGCTCGACACCGTTCGACTTCGACGGATTCCTCGGTCGTAAGCGGGCTGACGCCTCG
CGTGGCCGGTGA

Structures

Antitoxin

• AlphaFold DB: S1EJF5