Detailed information of TA system TA230856

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 7727..8307
• Replicon: plasmid p4
• Accession: NZ_CP090466
• Organism: Klebsiella pneumoniae strain kp5152

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: LZ688_RS29385
• Protein ID: WP_071177730.1
• Coordinates: 7727..8041 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: LZ688_RS29390
• Protein ID: WP_000093040.1
• Coordinates: 8029..8307 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ688_RS29360 (LZ688_29360)	3671..5635	+	1965	WP_071177727.1	TraM recognition domain-containing protein	-
LZ688_RS29365 (LZ688_29365)	5635..6366	+	732	WP_071177728.1	MobC family replication-relaxation protein	-
LZ688_RS29370 (LZ688_29370)	6373..6903	+	531	WP_071177729.1	hypothetical protein	-
LZ688_RS29375 (LZ688_29375)	6930..7109	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
LZ688_RS29380 (LZ688_29380)	7135..7563	-	429	WP_001140599.1	hypothetical protein	-
LZ688_RS29385 (LZ688_29385)	7727..8041	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LZ688_RS29390 (LZ688_29390)	8029..8307	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
LZ688_RS29395 (LZ688_29395)	8482..8847	-	366	WP_072354022.1	TonB family protein	-
LZ688_RS29400 (LZ688_29400)	8844..9215	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
LZ688_RS29405 (LZ688_29405)	9489..9734	-	246	WP_032440458.1	hypothetical protein	-
LZ688_RS29410 (LZ688_29410)	10379..11866	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11970	11970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T230856 WP_071177730.1 NZ_CP090466:c8041-7727 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T230856 NZ_CP125620:2181492-2181869 [Mycobacterium tuberculosis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT230856 WP_000093040.1 NZ_CP090466:c8307-8029 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT230856 NZ_CP125620:2181911-2182360 [Mycobacterium tuberculosis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1