Detailed information of TA system TA023085

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 67259..67784
• Replicon: plasmid pKP91
• Accession: NC_011281
• Organism: Klebsiella pneumoniae 342

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: KPK_RS27740
• Protein ID: WP_012539978.1
• Coordinates: 67479..67784 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: KPK_RS27735
• Protein ID: WP_012539986.1
• Coordinates: 67259..67477 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPK_RS27725	64084..65913	-	1830	WP_012539966.1	hypothetical protein	-
KPK_RS27730	66089..66673	-	585	WP_041165527.1	hypothetical protein	-
KPK_RS29870	66862..67050	+	189	Protein_66	hypothetical protein	-
KPK_RS27735	67259..67477	+	219	WP_012539986.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
KPK_RS27740	67479..67784	+	306	WP_012539978.1	type II toxin-antitoxin system toxin CcdB	Toxin
KPK_RS27745	67971..68975	+	1005	WP_029497484.1	hypothetical protein	-
KPK_RS27750	69173..69952	+	780	WP_029497485.1	site-specific integrase	-
KPK_RS27755	70010..70267	-	258	WP_000764642.1	hypothetical protein	-
KPK_RS31445	70396..70509	-	114	WP_012540012.1	hypothetical protein	-
KPK_RS27760	71143..71898	+	756	WP_012539983.1	replication initiation protein RepE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..91096	91096

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (3-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11601.27 Da
• Isoelectric Point: 6.4661

>T23085 WP_012539978.1 NC_011281:67479-67784 [Klebsiella variicola]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T23085 NC_011281:67479-67784 [Klebsiella variicola]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8362.27 Da
• Isoelectric Point: 4.5045

>AT23085 WP_012539986.1 NC_011281:67259-67477 [Klebsiella variicola]
MQQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIDMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT23085 NC_011281:67259-67477 [Klebsiella variicola]
ATGCAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGATATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA