Detailed information of TA system TA230848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 88482..89152
• Replicon: plasmid p1
• Accession: NZ_CP090463
• Organism: Klebsiella pneumoniae strain kp5152

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: LZ688_RS27545
• Protein ID: WP_004213072.1
• Coordinates: 88482..88925 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: LZ688_RS27550
• Protein ID: WP_004213073.1
• Coordinates: 88922..89152 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ688_RS27510 (LZ688_27510)	83893..84168	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
LZ688_RS27515 (LZ688_27515)	84231..84722	+	492	WP_011251327.1	DM13 domain-containing protein	-
LZ688_RS27520 (LZ688_27520)	84771..85691	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
LZ688_RS27525 (LZ688_27525)	85782..86185	+	404	Protein_99	GAF domain-containing protein	-
LZ688_RS27530 (LZ688_27530)	86703..87338	-	636	Protein_100	mucoid phenotype regulator RmpA2	-
LZ688_RS27535 (LZ688_27535)	87755..88059	+	305	Protein_101	transposase	-
LZ688_RS27540 (LZ688_27540)	88082..88333	-	252	WP_186987481.1	hypothetical protein	-
LZ688_RS27545 (LZ688_27545)	88482..88925	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
LZ688_RS27550 (LZ688_27550)	88922..89152	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LZ688_RS27555 (LZ688_27555)	89760..90893	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
LZ688_RS27560 (LZ688_27560)	90909..91202	+	294	WP_004213076.1	hypothetical protein	-
LZ688_RS27565 (LZ688_27565)	91192..91398	-	207	WP_004213077.1	hypothetical protein	-
LZ688_RS27570 (LZ688_27570)	91750..92040	+	291	WP_004213078.1	hypothetical protein	-
LZ688_RS27575 (LZ688_27575)	92030..92929	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..176459	176459

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T230848 WP_004213072.1 NZ_CP090463:c88925-88482 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T230848 NZ_CP125620:c1390486-1390193 [Mycobacterium tuberculosis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT230848 WP_004213073.1 NZ_CP090463:c89152-88922 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT230848 NZ_CP125620:c1390752-1390483 [Mycobacterium tuberculosis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620