Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 7916..8559 Replicon plasmid p1
Accession NZ_CP090463
Organism Klebsiella pneumoniae strain kp5152

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag LZ688_RS27080 Protein ID WP_001044770.1
Coordinates 8143..8559 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag LZ688_RS27075 Protein ID WP_001261282.1
Coordinates 7916..8146 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZ688_RS27050 (LZ688_27050) 3856..4023 - 168 Protein_3 IS1 family transposase -
LZ688_RS27055 (LZ688_27055) 4327..5256 + 930 WP_004213558.1 Rpn family recombination-promoting nuclease/putative transposase -
LZ688_RS27060 (LZ688_27060) 5401..6180 - 780 WP_004213560.1 site-specific integrase -
LZ688_RS27065 (LZ688_27065) 6177..6998 - 822 WP_004213562.1 hypothetical protein -
LZ688_RS27070 (LZ688_27070) 7543..7959 - 417 WP_164481821.1 hypothetical protein -
LZ688_RS27075 (LZ688_27075) 7916..8146 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LZ688_RS27080 (LZ688_27080) 8143..8559 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
LZ688_RS27085 (LZ688_27085) 8633..10195 + 1563 WP_004206609.1 AAA family ATPase -
LZ688_RS27090 (LZ688_27090) 10180..11202 + 1023 WP_000361404.1 helicase UvrD -
LZ688_RS27095 (LZ688_27095) 11458..12155 + 698 WP_223174630.1 IS1 family transposase -
LZ688_RS27100 (LZ688_27100) 12184..12456 + 273 Protein_13 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..176459 176459
- flank IS/Tn - - 2724..3704 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T230846 WP_001044770.1 NZ_CP090463:8143-8559 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T230846 NZ_CP125620:1241115-1241489 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT230846 WP_001261282.1 NZ_CP090463:7916-8146 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT230846 NZ_CP125620:1240921-1241118 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References