Detailed information of TA system TA230825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 83125..83650
• Replicon: plasmid pB379_3
• Accession: NZ_CP090458
• Organism: Escherichia coli strain B379

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: LZ684_RS24305
• Protein ID: WP_001159868.1
• Coordinates: 83345..83650 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: LZ684_RS24300
• Protein ID: WP_000813634.1
• Coordinates: 83125..83343 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ684_RS24270 (78517)	78517..78933	-	417	WP_001034046.1	type II toxin-antitoxin system VapC family toxin	-
LZ684_RS24275 (78930)	78930..79160	-	231	WP_001261278.1	type II toxin-antitoxin system VapB family antitoxin	-
LZ684_RS24280 (79425)	79425..79925	+	501	WP_000528931.1	HEPN family nuclease	-
LZ684_RS24285 (79938)	79938..80711	+	774	WP_000905949.1	hypothetical protein	-
LZ684_RS24290 (80878)	80878..82011	+	1134	WP_001542067.1	DUF3800 domain-containing protein	-
LZ684_RS24295 (82045)	82045..82533	-	489	WP_011254646.1	hypothetical protein	-
LZ684_RS24300 (83125)	83125..83343	+	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LZ684_RS24305 (83345)	83345..83650	+	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
LZ684_RS24310 (83651)	83651..84457	+	807	WP_000016982.1	site-specific integrase	-
LZ684_RS24315 (85231)	85231..85986	+	756	WP_000852146.1	replication initiation protein RepE	-
LZ684_RS24320 (86574)	86574..87740	+	1167	WP_000772446.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / blaCTX-M-14 / qacE / cmlA1 / sul2 / floR / erm(42) / ant(3'')-Ia / sitABCD	iucA / iucB / iucC / iucD / iutA	1..147204	147204

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T230825 WP_001159868.1 NZ_CP090458:83345-83650 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T230825 NZ_CP125620:71859-72260 [Mycobacterium tuberculosis]
GTGGATGAATGTGTAGTCGACGCGGCGGCCGTGGTTGACGCTCTCGCCGGCAAGGGCGCCAGCGCGATCGTTCTGCGCGG
TTTGCTCAAGGAGTCGATTTCTAACGCGCCGCATTTGCTGGACGCAGAGGTCGGACATGCACTCCGCCGCGCCGTGCTCA
GCGACGAAATCTCCGAAGAGCAGGCTCGCGCCGCGTTGGATGCCTTGCCTTATCTCATCGACAATCGTTACCCGCACAGC
CCACGACTGATCGAATACACATGGCAGCTAAGGCACAACGTCACGTTCTACGACGCCCTTTACGTCGCACTGGCCACCGC
ACTGGATGTCCCGCTGCTCACGGGCGACTCGCGGCTTGCGGCCGCGCCGGGCCTTCCGTGCGAAATCAAACTCGTTCGGT
GA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT230825 WP_000813634.1 NZ_CP090458:83125-83343 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT230825 NZ_CP125620:71627-71866 [Mycobacterium tuberculosis]
ATGGCTACCATTCAAGTTCGGGATTTGCCCGAAGATGTCGCCGAAACCTATCGACGGCGCGCCACCGCAGCGGGGCAGTC
GCTGCAGACGTATATGCGCACCAAGCTCATCGAAGGGGTGCGGGGCCGAGACAAGGCCGAGGCAATCGAGATCCTGGAAC
AGGCGCTCGCCAGCACTGCCAGCCCAGGCATCAGCCGGGAGACCATCGAGGCATCCCGGCGGGAGCTCAGGGGTGGATGA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2