Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 73688..74331 Replicon plasmid pB379_3
Accession NZ_CP090458
Organism Escherichia coli strain B379

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag LZ684_RS24255 Protein ID WP_001034044.1
Coordinates 73688..74104 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag LZ684_RS24260 Protein ID WP_001261286.1
Coordinates 74101..74331 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZ684_RS24240 (70090) 70090..70787 + 698 WP_095033700.1 IS1-like element IS1B family transposase -
LZ684_RS24245 (71041) 71041..72063 - 1023 WP_000361402.1 helicase UvrD -
LZ684_RS24250 (72048) 72048..73613 - 1566 WP_001128474.1 AAA family ATPase -
LZ684_RS24255 (73688) 73688..74104 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
LZ684_RS24260 (74101) 74101..74331 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LZ684_RS24265 (74678) 74678..78472 + 3795 WP_001144732.1 hypothetical protein -
LZ684_RS24270 (78517) 78517..78933 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
LZ684_RS24275 (78930) 78930..79160 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid erm(B) / blaCTX-M-14 / qacE / cmlA1 / sul2 / floR / erm(42) / ant(3'')-Ia / sitABCD iucA / iucB / iucC / iucD / iutA 1..147204 147204
- flank IS/Tn - - 70410..70787 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T230823 WP_001034044.1 NZ_CP090458:c74104-73688 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T230823 NZ_CP125619:c2423398-2423090 [Pseudomonas sp. PMCC200367]
ATGAGCCAGACTTTGACTACTTTCGATATGGCGGCTCTGCTGGACAGTGACGAAGCCATCAGCGAGTATCTTTCGCAAGT
GCTCGCAGACGGCGACAACGAAGAATTTCTTCGCGCTATCGGCTATGTGGTGAAAGCGCGTGGAATGACACAGATAGCCA
AGGATTCGGGTATGGGCCGCGAAAGCCTGTACAAGGCCTTCGCACCAGGTGCAAAACCACGTTTCGATACGGTTTTAAAG
GTCATTCACGCCCTTGGCATTGATCTCTGCGCGCAGCCGGGTCATTCGGTCAATCACTCGACTCTCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT230823 WP_001261286.1 NZ_CP090458:c74331-74101 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT230823 NZ_CP125619:c2423694-2423401 [Pseudomonas sp. PMCC200367]
ATGAATTATCTTATTCAACAAACTATGATCTTCGCGACATGGCATGCCTCAGTTCGTGACTTGCGGGCGAAGTTAGCCAT
CGCTCGTCGCATTGATCGTGCCTCTGCGGGTAATCTGGGCGATATCAAACCGGTGGGTGATGGTGTTTCCGAAATGCGCG
TGGACGTCGGTGCAGGCTATCGGGTCTACTTCACTATGCGAAATTGCGTCGTCATTGTGCTGTTGGCAGGTGGTGACAAA
TCTTCGCAAACCGCCGACATCAGGCGGGCGAAAAAATTGGCGAAGGAGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References