Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 44867..45426 Replicon plasmid pB379_3
Accession NZ_CP090458
Organism Escherichia coli strain B379

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag LZ684_RS24105 Protein ID WP_000421272.1
Coordinates 45151..45426 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag LZ684_RS24100 Protein ID WP_001387467.1
Coordinates 44867..45151 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZ684_RS24070 (39938) 39938..40776 - 839 Protein_46 AadA family aminoglycoside 3''-O-nucleotidyltransferase -
LZ684_RS24075 (40925) 40925..41938 + 1014 WP_000845048.1 class 1 integron integrase IntI1 -
LZ684_RS24080 (42141) 42141..42377 + 237 Protein_48 DUF3330 domain-containing protein -
LZ684_RS24085 (42430) 42430..43134 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
LZ684_RS24090 (43195) 43195..43464 + 270 Protein_50 2-keto-3-deoxygluconate permease -
LZ684_RS24095 (43510) 43510..44262 - 753 WP_000562172.1 IclR family transcriptional regulator -
LZ684_RS24100 (44867) 44867..45151 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
LZ684_RS24105 (45151) 45151..45426 + 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LZ684_RS24110 (45532) 45532..45735 + 204 Protein_54 hypothetical protein -
LZ684_RS24115 (45860) 45860..45988 - 129 Protein_55 IS481 family transposase -
LZ684_RS24120 (46277) 46277..47191 - 915 WP_223429136.1 hypothetical protein -
LZ684_RS24130 (49059) 49059..49327 + 269 Protein_58 type II toxin-antitoxin system ParD family antitoxin -
LZ684_RS24135 (49314) 49314..49603 + 290 Protein_59 type II toxin-antitoxin system RelE/ParE family toxin -
LZ684_RS24140 (49670) 49670..49852 + 183 WP_042344623.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid erm(B) / blaCTX-M-14 / qacE / cmlA1 / sul2 / floR / erm(42) / ant(3'')-Ia / sitABCD iucA / iucB / iucC / iucD / iutA 1..147204 147204
- inside IScluster/Tn erm(B) / blaCTX-M-14 / qacE / cmlA1 / sul2 / floR / erm(42) / ant(3'')-Ia / sitABCD - 1105..58407 57302


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T230822 WP_000421272.1 NZ_CP090458:45151-45426 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T230822 NZ_CP125619:1886026-1886238 [Pseudomonas sp. PMCC200367]
GTGCAGAGCAGGCTATTGATCAAGGAGTTGGAGGAGGCCGGGTGGATACTGGATCGGATTACCGGGAGCCATCACCTCTT
CAAACACCGATACAACCCGTACACGATACCCGTCCCTCATCCGAAAAAGGATTTGCCGATAGGGACGGTCAAAAGCATCA
GGAGGCGAGCCGGGTTGTATTGCCCGGGAGCCAGTTATGCAGGAGATCCATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT230822 WP_001387467.1 NZ_CP090458:44867-45151 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT230822 NZ_CP125619:1886238-1886642 [Pseudomonas sp. PMCC200367]
ATGCAATATCCAATCTGTATCGAGTGGGGCGACGAGAACACCGCCATCGGTATTCAGATCCCCGATATTCCAGGCGCCGT
AACGGCCGGGGATACGTTTGAAGACGCCTATAACGCGGCGGTCGAGATTGCTCACATCATGCTTCAGGAGATTGCGGCTG
ACGGGGAGTCGATTCCCATGCCTACGTCTGCTGCCGTTCACCGTGGCAATCCCGACTTTGCCGACATGGGCTGGGGAATG
CTTGAGCTGGACATCGCGCCGTACATGGGCAAAACCGAGAAGGTCAATGTGACGCTGCCCGGCTATGTGATTCAGCGCAT
TGATCGATATGTGCGCGAGCACAATGTCAAAAGCCGCTCTTCCTTTCTGGCAGATGCGGCGATGGAGAAGTTGGTTCGAC
ACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References