Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 71711..72509 Replicon plasmid pB379_2
Accession NZ_CP090457
Organism Escherichia coli strain B379

Toxin (Protein)


Gene name ataT Uniprot ID A0A2L1KTV6
Locus tag LZ684_RS23380 Protein ID WP_023135659.1
Coordinates 71988..72509 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A141BRA2
Locus tag LZ684_RS23375 Protein ID WP_001711191.1
Coordinates 71711..71980 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZ684_RS23350 (LZ684_23350) 67138..68478 - 1341 WP_023135655.1 DnaB-like helicase C-terminal domain-containing protein -
LZ684_RS23355 (LZ684_23355) 68539..69264 - 726 WP_023135656.1 hypothetical protein -
LZ684_RS23360 (LZ684_23360) 69547..70314 + 768 WP_001711193.1 hypothetical protein -
LZ684_RS23365 (LZ684_23365) 70367..70720 - 354 WP_161953766.1 hypothetical protein -
LZ684_RS23370 (LZ684_23370) 70726..71391 - 666 WP_023135658.1 AAA family ATPase -
LZ684_RS23375 (LZ684_23375) 71711..71980 + 270 WP_001711191.1 DUF1778 domain-containing protein Antitoxin
LZ684_RS23380 (LZ684_23380) 71988..72509 + 522 WP_023135659.1 GNAT family N-acetyltransferase Toxin
LZ684_RS23385 (LZ684_23385) 72678..72929 - 252 WP_023135660.1 hypothetical protein -
LZ684_RS23390 (LZ684_23390) 72932..73699 - 768 WP_023135661.1 hypothetical protein -
LZ684_RS23395 (LZ684_23395) 73722..75296 - 1575 WP_095526113.1 hypothetical protein -
LZ684_RS23400 (LZ684_23400) 75330..76586 - 1257 WP_023135663.1 phage terminase large subunit -
LZ684_RS23405 (LZ684_23405) 76589..77230 - 642 WP_023135664.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..152061 152061


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(48-149)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19382.22 Da        Isoelectric Point: 8.2535

>T230821 WP_023135659.1 NZ_CP090457:71988-72509 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKETLPSKSQQ
KKVPYRHVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKSLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Download         Length: 522 bp

>T230821 NZ_CP125619:c1550066-1549782 [Pseudomonas sp. PMCC200367]
ATGCGGGTTGAGTGGCTGCGTACTGCATTAAAGAATCTCGATGACGAAGCCGCGTACATCGCGATGGAAAACCCAAAGGC
GGCGGCAGATTTCGTTCAAGCCATTCTCGCCAACGTTGATCACCTTGCTCGATTTCCCGCGACAGGCCGAGAAGGTCGCT
TGCCCGGGACTCGTGAATGGGTTTTGCCCGACCGGCCGTATCTGATTCCCTATCGAGTTCGCCAAGGTCGCTTGCAAGCG
CTTAGGGTCTTCCATACTCGGCGACTTCCCCCCACCAATTGGTAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10054.53 Da        Isoelectric Point: 8.7353

>AT230821 WP_001711191.1 NZ_CP090457:71711-71980 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Download         Length: 270 bp

>AT230821 NZ_CP125619:c1550295-1550056 [Pseudomonas sp. PMCC200367]
ATGTCCGTCATGTCTTTACGCTTGCCAGACGAACTGGCCGATACGCTCGCCAACCTTGCCAAGGCCACCGGTCGCAGCAA
GTCATTTCTCGCGGTGGATGCTCTGCGTGAGTATCTCGCGCGAGAAGCCTGGCAAATCGAGGAAATTCAGAAAGCGCTGA
AAGAAGCCGATGCCGGTGACTTCGCAACGCCGGAAGAAGTGAATGCAATCGCCGACAAGTGGACAGCCAATGCGGGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1KTV6

Antitoxin

Source ID Structure
AlphaFold DB A0A141BRA2

References