Detailed information of TA system TA230819

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4123261..4123940
• Replicon: chromosome
• Accession: NZ_CP090456
• Organism: Escherichia coli strain B379

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: LZ684_RS19825
• Protein ID: WP_234679202.1
• Coordinates: 4123261..4123602 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: LZ684_RS19830
• Protein ID: WP_234679204.1
• Coordinates: 4123623..4123940 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ684_RS19800 (4118556)	4118556..4119662	+	1107	WP_001315214.1	N-acetylneuraminate epimerase	-
LZ684_RS19805 (4119727)	4119727..4120707	+	981	WP_001295601.1	sialate O-acetylesterase	-
LZ684_RS19810 (4120715)	4120715..4121365	-	651	WP_001037966.1	HNH endonuclease	-
LZ684_RS19815 (4121502)	4121502..4121645	+	144	Protein_3878	HNH endonuclease	-
LZ684_RS19820 (4122481)	4122481..4123125	+	645	WP_050488383.1	hypothetical protein	-
LZ684_RS19825 (4123261)	4123261..4123602	-	342	WP_234679202.1	TA system toxin CbtA family protein	Toxin
LZ684_RS19830 (4123623)	4123623..4123940	-	318	WP_234679204.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LZ684_RS19835 (4123961)	4123961..4124182	-	222	WP_234679206.1	DUF987 domain-containing protein	-
LZ684_RS19840 (4124191)	4124191..4124667	-	477	WP_222563671.1	RadC family protein	-
LZ684_RS19845 (4124683)	4124683..4125141	-	459	WP_222563670.1	antirestriction protein	-
LZ684_RS19850 (4125243)	4125243..4125482	-	240	WP_234679208.1	DUF905 domain-containing protein	-
LZ684_RS19855 (4125559)	4125559..4126026	-	468	WP_136392182.1	protein YkfB	-
LZ684_RS19860 (4126050)	4126050..4126448	-	399	WP_234679210.1	hypothetical protein	-
LZ684_RS19865 (4126629)	4126629..4128170	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
LZ684_RS19870 (4128185)	4128185..4128931	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimE / fimB	4114691..4149844	35153

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12852.91 Da
• Isoelectric Point: 8.0308

>T230819 WP_234679202.1 NZ_CP090456:c4123602-4123261 [Escherichia coli]
MKTLPATISRATKPCLSPVAVWQMLLTRLLEQHYGLTLDDTAFSDETVIKEHIDAGITLADAVNFLVDKYELVRIDRRGF
SWQEQSPYLRAVDILRARRAIGLLRRSINDAVL

Nucleotide

• Length: 342 bp

>T230819 NZ_CP125619:c1149362-1149108 [Pseudomonas sp. PMCC200367]
ATGAATATCGAGTTCACTCCTGAAGGTTGGGAAGACTATCTGTGGTTCCAACAAAACGATAAGGCAGGACTCAAGCGAAT
CAACCTGCTGATCAAATCAATCCAGCGCGAGCCGTTCGATGGACTCGGAAAACCTGAACCGCTCAAATACAACTTGAGCG
GCTTCTGGTCGCGCCGGATTACAGCCGAGCATCGCTTGGTCTATGCAGTCGAGGATGGTGAAATCCGCGTTGTGATGTGT
AGATATCACTACTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12088.73 Da
• Isoelectric Point: 7.8418

>AT230819 WP_234679204.1 NZ_CP090456:c4123940-4123623 [Escherichia coli]
MTHNTWGLQRAITPRLGARLVQERNRLHYLADRASITGKFSDAEYRKLDETFPHFIRQMESMLTTAELSPQHAHCVILYH
NGFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 318 bp

>AT230819 NZ_CP125619:c1149613-1149359 [Pseudomonas sp. PMCC200367]
ATGCAAACCATCAACTACACCACCGCACGTGCACATCTGGCCGAAACCATGGATCGGGTCAATGAAGACCGCGCTCCACT
GTTGGTCACTCGCCAGAAAGGCGAACCCGTGGTGATGATGTCTCTGGCGGAGTACAACGCCCTCGAAGAGACAGCCTATC
TACTTCGCTCCCCTGCTAATGCAGAACGCTTGATCAAATCGATCGGCAACCTGCGAGCAGGGAAAACTAAAACCAGGCAA
CTCATCGAAGAATGA