Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4087100..4087676 Replicon chromosome
Accession NZ_CP090456
Organism Escherichia coli strain B379

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag LZ684_RS19640 Protein ID WP_001297643.1
Coordinates 4087389..4087676 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag LZ684_RS19635 Protein ID WP_000063156.1
Coordinates 4087100..4087402 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZ684_RS19620 (4083696) 4083696..4085846 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
LZ684_RS19625 (4085896) 4085896..4086099 + 204 WP_000467859.1 YbdD/YjiX family protein -
LZ684_RS19630 (4086110) 4086110..4087066 + 957 WP_001297640.1 GTPase -
LZ684_RS19635 (4087100) 4087100..4087402 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
LZ684_RS19640 (4087389) 4087389..4087676 - 288 WP_001297643.1 BrnT family toxin Toxin
LZ684_RS19645 (4087875) 4087875..4088572 - 698 Protein_3844 IS1 family transposase -
LZ684_RS19650 (4088629) 4088629..4088724 + 96 Protein_3845 dienelactone hydrolase family protein -
LZ684_RS19655 (4088870) 4088870..4090102 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
LZ684_RS19660 (4090143) 4090143..4091423 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T230818 WP_001297643.1 NZ_CP090456:c4087676-4087389 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T230818 NZ_CP125619:473920-474216 [Pseudomonas sp. PMCC200367]
ATGCATGTGTTCACCGAAAAACCAATCCATGAAGCCAAGGAAAAGTGGCCGCGGGCAGCCAATGCACTGGAGCAGTGGTA
TCGACTTGCCAAAGGGAGTAACCCGGGAGATTTCGCGGCGATGAAGTCGATTTTCCCCGCGACTGACAAAGTCGGCGAGT
TTCATGTGTTCGACATTGGTGGCAACAAGTTGCGGCTGATCGCTTTTGTCAGGTATCGGTCTCAAAAGCTTTATATAAAG
CATGTGCTGGATCACCGCGAATATGACCGCGGTAAATGGAAGGAGCTAAGGGCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT230818 WP_000063156.1 NZ_CP090456:c4087402-4087100 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT230818 NZ_CP125619:474213-474614 [Pseudomonas sp. PMCC200367]
ATGAGTGTTCTGATCAAAAAAGCTGCCGAGCATTGGGAGTTCGTCTCGCCATTGCTTCGAAAACCGAAAAACGAAGACGA
CTATGACATTTTGGCGCAGGCACTTGATGAATTGCTTGAGATGACCGGGGATGATGAGTCGCACCCGTTGATGAGCTTGG
TGGACATCATCGGCGACTGGATTGCAGAGTGGGATCGTGAGCATCACCCCATGCGCGAGGCCTCTGGTGCAGAGGTTCTC
GGCTACATGATGCGCGAACACGGATTGAGCCAAAGCGATCTGCCCGGCGTCGGCACCCAATCGGTGGTATCCGAGATCTT
GAGCGGCAAGCGTCAGCTCAACCTGCGGCAGATCCGCTGGTTGGCCGAGCGCTTCGGAGTGTCGGTGGAGACCTTTATCT
GA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References