Detailed information of TA system TA230798

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 7045..7592
• Replicon: plasmid pPT62-37kb
• Accession: NZ_CP090452
• Organism: Escherichia coli strain PT62

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: LZ175_RS23890
• Protein ID: WP_244578848.1
• Coordinates: 7314..7592 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A829FT18
• Locus tag: LZ175_RS23885
• Protein ID: WP_000079938.1
• Coordinates: 7045..7314 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ175_RS23850 (LZ175_23840)	2340..2630	-	291	WP_001471781.1	hypothetical protein	-
LZ175_RS23855 (LZ175_23845)	3289..3624	+	336	WP_001039462.1	plasmid stabilization protein StbA	-
LZ175_RS23860 (LZ175_23850)	3633..3824	+	192	WP_001515192.1	hypothetical protein	-
LZ175_RS23865 (LZ175_23855)	3835..4418	-	584	Protein_5	IS1 family transposase	-
LZ175_RS23870 (LZ175_23860)	4952..5707	+	756	WP_000807690.1	replication initiation protein RepE	-
LZ175_RS23875 (LZ175_23865)	5976..6321	+	346	Protein_7	IS1 family transposase	-
LZ175_RS23880 (LZ175_23870)	6832..6972	+	141	WP_160784086.1	hypothetical protein	-
LZ175_RS23885 (LZ175_23875)	7045..7314	+	270	WP_000079938.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
LZ175_RS23890 (LZ175_23880)	7314..7592	+	279	WP_244578848.1	type II toxin-antitoxin system toxin YacB	Toxin
LZ175_RS23895 (LZ175_23885)	7628..9379	-	1752	Protein_11	Tn3-like element TnAs1 family transposase	-
LZ175_RS23900 (LZ175_23890)	9383..9775	+	393	WP_001351729.1	isochorismatase family cysteine hydrolase	-
LZ175_RS23905 (LZ175_23895)	9913..10797	+	885	WP_000058717.1	EamA family transporter	-
LZ175_RS23910 (LZ175_23900)	10829..12028	-	1200	WP_001493765.1	tetracycline efflux MFS transporter Tet(A)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(A) / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / sul3	-	1..37779	37779
	-	inside	IScluster/Tn	tet(A) / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / sul3	-	1250..34407	33157

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10729.39 Da
• Isoelectric Point: 8.4615

>T230798 WP_244578848.1 NZ_CP090452:7314-7592 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMVAIELDERIGYSASSLAGQPYKGRNGRVEGARELVIHPHFVLVYEVDSPWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T230798 NZ_CP125367:1636890-1637759 [Pseudomonas aeruginosa]
ATGAACATGGCGCAGACCCACACCCCCGTCCCGAACCTGCAACAGCGCTTTCCCGAGCGGCTGGTGCAACTGGCCGACGG
CGCCCAGCTGGCCATCCGCGAATGCGGCCAGGGCCCGGTGGTGGTGCTGTTGCACGGCATCGGTTCGGGCTCGGCGTCCT
GGCTGCATTGCGCCCAGCGCCTGGCCGCCGGCAATCGGGTGATCGCCTGGGACGCGCCGGGTTACGGCCTGTCCACGCCG
CTGCCGCCGGCCCGGCCCAAGGCCTGCGACTACGCCGCGTGCCTGGAGCTGCTGCTGGATGCGCTGGGCGTGGAGAGCTG
CCTGCTGGTGGGCCACTCCCTCGGCGCGCTGATGGCCACGGCCTACGCCGCCGGCATCGGCGCGGCGCGGGTGCGCCGCC
TGGTGCTGCTCAGCCCGGCGCGCGGCTACGGCGCCGCCGAGCTGCGCGACAGCGGCGCCCAGGTGCGCCGCCAGCGCCTG
GAGAACCTGGAGCGCTTCGGTATCGACGGCATGGCCAGCGAGCGCACCGCGCGCCTGCTCGGCCGCAACCCCAGCGAGGA
GGCCCTGGCCTGGGTGCGCTGGAACATGGCGCGGCTCAACCCCGAGGGCTACCGCCAGGCCGTCGAGCTGCTCTGCGGCG
ACGACCTGCTGGGCAACGGGCAGCCTGCCGCTCCTTGCGAGGTGCATTGCGGCGAGGACGACGGCATCACCACGCCCGAG
AGTTGCGGCGCCATCGCCCGCCAGCTCGGCGCGTCGTTCTCGTCGATTCCCGGGGCCGGCCACGCCAGCCCGATCGAACA
ACCCGAAGTGGTCGCAGGACGCATCGGCCACGCGCAACGCCTCTCTCTTGAAGGAACCGCCAATGGATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10251.64 Da
• Isoelectric Point: 10.1734

>AT230798 WP_000079938.1 NZ_CP090452:7045-7314 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLKRGDVLPHDMVESSAAAWRD
EMSRKVADK

Nucleotide

• Length: 270 bp

>AT230798 NZ_CP125367:1637752-1638585 [Pseudomonas aeruginosa]
ATGGATAAGTCAGACGATTCGCAAGACAAGTACATCGTGCCTGGCCTCGAACGCGGCCTGCTGCTGCTCTGCGAGTTCAG
CCGGAAGGACCGTACCCTGACGGCGCCGGAGTTGGCGCGGCGCCTGAAGCTGCCGCGCTCGACCATCTTCCGCCTGCTGA
CCACCCTGGAGGCCATGGGCTTCGTCACCCGCAACGGCAACGAGTACCGCCTGGGCATGGCGGTGTTGCGCCTGGGCTTC
GAGTACCTGGCCTCGCTGGAGCTGACCGAACTCGGCCAGCCGCTGCTGAACCGCCTGTGCGACGAGATCCGCTACCCCTG
CAACCTGGTGGTGCGCGACGGCCGTTCGATCGTCTACGTGGCCAAGGTTTCGCCGTCCACCCCGCTGAGCAGCTCGGTGA
ACGTCGGGACCCGGTTGCCGGCCCACGCCACGGTGCTCGGCCGCATCCTGCTGCAGGACCTGAGCCTGGGCGAGCTGCGC
GAGCTGTACCCGGAGGAGCAGCTGGAGCAGTTCTCGCCGAACACCCCGCGCAGCGTGCTGGAACTCTTCGACATGGTCCA
GGGCGACCGCCAGCGTGGCTTCGTCCAGGGCGAGGGGTTCTTCGAGGCGAGCATCTCCACCGTCGCGGCGCCGGTACGTG
ATCACAGCGGTCGCGTCATCGCGGCGATGGGGGCGACCATCGCCGCCGGGCATATCGACCCGGAACGCATCGAGGGACTG
GTCAGCCGGGTGCGCAGCAGCGCCGACGAGCTGTCGTACCTGCTCGACTACCGCGCCGACGGCCAGGACAACGTCACCCC
GATCTTCCGGAGTCGCCCGCATGAAACCGTATGA

Structures

Antitoxin

• AlphaFold DB: A0A829FT18