Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 97878..98479 Replicon plasmid pPT62-106kb
Accession NZ_CP090450
Organism Escherichia coli strain PT62

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag LZ175_RS23450 Protein ID WP_001216034.1
Coordinates 98099..98479 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag LZ175_RS23445 Protein ID WP_001190712.1
Coordinates 97878..98099 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZ175_RS23435 (94859) 94859..96142 - 1284 WP_001617890.1 restriction endonuclease subunit S -
LZ175_RS23440 (96139) 96139..97695 - 1557 WP_001617892.1 type I restriction-modification system subunit M -
LZ175_RS23445 (97878) 97878..98099 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LZ175_RS23450 (98099) 98099..98479 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LZ175_RS23455 (98484) 98484..98663 + 180 WP_001513661.1 hypothetical protein -
LZ175_RS23460 (98691) 98691..98969 + 279 Protein_118 pdcB -
LZ175_RS23465 (98974) 98974..99387 + 414 Protein_119 integrase core domain-containing protein -
LZ175_RS23470 (99337) 99337..99672 - 336 WP_169329198.1 type I deoxyribonuclease HsdR -
LZ175_RS23475 (99882) 99882..100860 - 979 Protein_121 IS5-like element IS5 family transposase -
LZ175_RS24120 (101164) 101164..101818 + 655 Protein_122 amino acid permease -
LZ175_RS24125 (101928) 101928..102287 + 360 WP_265180582.1 hypothetical protein -
LZ175_RS23485 (102292) 102292..102504 + 213 WP_234630904.1 hypothetical protein -
LZ175_RS23490 (102491) 102491..103419 + 929 Protein_125 homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / mef(B) / sul3 - 1..106761 106761


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T230797 WP_001216034.1 NZ_CP090450:98099-98479 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T230797 NZ_CP125367:c176388-176107 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACACCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT230797 WP_001190712.1 NZ_CP090450:97878-98099 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT230797 NZ_CP125367:c176612-176385 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References