230796

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 83902..84545 Replicon plasmid pPT62-106kb
Accession NZ_CP090450
Organism Escherichia coli strain PT62

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag LZ175_RS23395 Protein ID WP_001044768.1
Coordinates 84129..84545 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag LZ175_RS23390 Protein ID WP_001261287.1
Coordinates 83902..84132 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZ175_RS23370 (79082) 79082..80215 - 1134 WP_000545983.1 DUF3800 domain-containing protein -
LZ175_RS23375 (80478) 80478..82115 - 1638 WP_223201785.1 hypothetical protein -
LZ175_RS23380 (82166) 82166..82996 - 831 WP_223201786.1 hypothetical protein -
LZ175_RS23385 (83048) 83048..83596 - 549 WP_039002808.1 hypothetical protein -
LZ175_RS23390 (83902) 83902..84132 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LZ175_RS23395 (84129) 84129..84545 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
LZ175_RS23400 (84707) 84707..86845 - 2139 WP_039002805.1 AAA family ATPase -
LZ175_RS23405 (87310) 87310..88227 + 918 WP_230069318.1 nucleotidyltransferase -
LZ175_RS23410 (88303) 88303..88643 + 341 Protein_108 transposase -
LZ175_RS23415 (88838) 88838..89527 + 690 WP_234630906.1 integrase core domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / mef(B) / sul3 - 1..106761 106761


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T230796 WP_001044768.1 NZ_CP090450:84129-84545 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T230796 NZ_CP125365:c5962025-5961747 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTTGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT230796 WP_001261287.1 NZ_CP090450:83902-84132 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT230796 NZ_CP125365:c5961735-5961430 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References