Detailed information of TA system TA230781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3814159..3814709
• Replicon: chromosome
• Accession: NZ_CP090448
• Organism: Escherichia coli strain PT62

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: LZ175_RS18615
• Protein ID: WP_001160975.1
• Coordinates: 3814159..3814473 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: LZ175_RS18620
• Protein ID: WP_000125566.1
• Coordinates: 3814476..3814709 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ175_RS18590 (3809834)	3809834..3811120	+	1287	WP_032222109.1	peptidylprolyl isomerase SurA	-
LZ175_RS18595 (3811120)	3811120..3812109	+	990	WP_044189225.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
LZ175_RS18600 (3812106)	3812106..3812927	+	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
LZ175_RS18605 (3812930)	3812930..3813307	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
LZ175_RS18610 (3813314)	3813314..3814162	+	849	WP_000257188.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
LZ175_RS18615 (3814159)	3814159..3814473	-	315	WP_001160975.1	CcdB family protein	Toxin
LZ175_RS18620 (3814476)	3814476..3814709	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LZ175_RS18625 (3814795)	3814795..3815274	-	480	WP_001487721.1	type 3 dihydrofolate reductase	-
LZ175_RS18630 (3815466)	3815466..3815930	-	465	Protein_3646	NAD-binding protein	-
LZ175_RS18640 (3816739)	3816739..3816957	+	219	WP_000196529.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3815945..3816448	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T230781 WP_001160975.1 NZ_CP090448:c3814473-3814159 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T230781 NZ_CP125363:c176389-176108 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACACCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT230781 WP_000125566.1 NZ_CP090448:c3814709-3814476 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT230781 NZ_CP125363:c176613-176386 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9