Detailed information of TA system TA230779

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3534801..3535480
• Replicon: chromosome
• Accession: NZ_CP090448
• Organism: Escherichia coli strain PT62

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: LZ175_RS17135
• Protein ID: WP_000854680.1
• Coordinates: 3535139..3535480 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: LZ175_RS17130
• Protein ID: WP_000070396.1
• Coordinates: 3534801..3535118 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ175_RS17085 (3530239)	3530239..3531060	+	822	WP_230069315.1	DUF932 domain-containing protein	-
LZ175_RS17090 (3531277)	3531277..3531978	+	702	WP_001581462.1	WYL domain-containing protein	-
LZ175_RS17095 (3532019)	3532019..3532255	+	237	WP_001144031.1	protein YpjK	-
LZ175_RS17100 (3532255)	3532255..3532698	+	444	WP_032199444.1	lipoprotein YafY	-
LZ175_RS17105 (3532721)	3532721..3533188	+	468	WP_032200803.1	protein YkfB	-
LZ175_RS17110 (3533265)	3533265..3533504	+	240	WP_180513156.1	DUF905 domain-containing protein	-
LZ175_RS17115 (3533602)	3533602..3534060	+	459	WP_032177818.1	antirestriction protein	-
LZ175_RS17120 (3534076)	3534076..3534552	+	477	WP_072667534.1	RadC family protein	-
LZ175_RS17125 (3534561)	3534561..3534782	+	222	WP_000691994.1	DUF987 domain-containing protein	-
LZ175_RS17130 (3534801)	3534801..3535118	+	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
LZ175_RS17135 (3535139)	3535139..3535480	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
LZ175_RS17140 (3536024)	3536024..3536224	+	201	Protein_3356	integrase	-
LZ175_RS17145 (3536291)	3536291..3537453	+	1163	WP_085947771.1	IS3-like element IS3 family transposase	-
LZ175_RS17150 (3537483)	3537483..3538562	+	1080	Protein_3358	tyrosine-type recombinase/integrase	-
LZ175_RS17155 (3538643)	3538643..3539353	-	711	WP_230069332.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3533602..3607111	73509

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T230779 WP_000854680.1 NZ_CP090448:3535139-3535480 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T230779 NZ_CP125361:c5745429-5745130 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT230779 WP_000070396.1 NZ_CP090448:3534801-3535118 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT230779 NZ_CP125361:c5745133-5744843 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7