Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3386872..3387709 Replicon chromosome
Accession NZ_CP090448
Organism Escherichia coli strain PT62

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag LZ175_RS16430 Protein ID WP_000227784.1
Coordinates 3387167..3387709 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag LZ175_RS16425 Protein ID WP_001297137.1
Coordinates 3386872..3387183 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZ175_RS16400 (3381892) 3381892..3382839 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
LZ175_RS16405 (3382861) 3382861..3384852 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
LZ175_RS16410 (3384842) 3384842..3385456 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
LZ175_RS16415 (3385456) 3385456..3385785 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
LZ175_RS16420 (3385797) 3385797..3386687 + 891 WP_000971336.1 heme o synthase -
LZ175_RS16425 (3386872) 3386872..3387183 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
LZ175_RS16430 (3387167) 3387167..3387709 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
LZ175_RS16435 (3387765) 3387765..3388700 - 936 WP_001371638.1 tetratricopeptide repeat protein -
LZ175_RS16440 (3389108) 3389108..3390472 + 1365 WP_001000954.1 MFS transporter -
LZ175_RS16445 (3390600) 3390600..3391091 - 492 WP_040062457.1 nucleotide binding protein YajQ -
LZ175_RS16450 (3391259) 3391259..3392170 + 912 WP_000705865.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T230778 WP_000227784.1 NZ_CP090448:3387167-3387709 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T230778 NZ_CP125361:c5745133-5744843 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT230778 WP_001297137.1 NZ_CP090448:3386872-3387183 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT230778 NZ_CP125361:c5745429-5745130 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References