Detailed information of TA system TA230765

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 993297..993880
• Replicon: chromosome
• Accession: NZ_CP090448
• Organism: Escherichia coli strain PT62

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LZ175_RS04795
• Protein ID: WP_089601808.1
• Coordinates: 993545..993880 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A161R357
• Locus tag: LZ175_RS04790
• Protein ID: WP_040062758.1
• Coordinates: 993297..993545 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ175_RS04780 (989636)	989636..990937	+	1302	WP_040062759.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
LZ175_RS04785 (990985)	990985..993219	+	2235	WP_000226815.1	GTP pyrophosphokinase	-
LZ175_RS04790 (993297)	993297..993545	+	249	WP_040062758.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
LZ175_RS04795 (993545)	993545..993880	+	336	WP_089601808.1	endoribonuclease MazF	Toxin
LZ175_RS04800 (993951)	993951..994742	+	792	WP_001071643.1	nucleoside triphosphate pyrophosphohydrolase	-
LZ175_RS04805 (994970)	994970..996607	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
LZ175_RS04810 (996695)	996695..997993	+	1299	WP_000036723.1	phosphopyruvate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• Antitoxin PemI-like (2-82)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12142.09 Da
• Isoelectric Point: 8.2618

>T230765 WP_089601808.1 NZ_CP090448:993545-993880 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKSSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTLAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T230765 NZ_CP125352:47814-48095 [Escherichia fergusonii]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9297.70 Da
• Isoelectric Point: 4.5972

>AT230765 WP_040062758.1 NZ_CP090448:993297-993545 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNGITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT230765 NZ_CP125352:47573-47824 [Escherichia fergusonii]
ATGAGCTATCAAATTCTGACAACTACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A161R357