Detailed information of TA system TA230756

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1243..1717
• Replicon: plasmid pE
• Accession: NZ_CP090437
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KPN857

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: LZ608_RS29295
• Protein ID: WP_004197762.1
• Coordinates: 1451..1717 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: LZ608_RS29290
• Protein ID: WP_000879771.1
• Coordinates: 1243..1467 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ608_RS29285 (LZ608_29285)	364..576	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
LZ608_RS29290 (LZ608_29290)	1243..1467	+	225	WP_000879771.1	DUF6290 family protein	Antitoxin
LZ608_RS29295 (LZ608_29295)	1451..1717	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LZ608_RS29300 (LZ608_29300)	1787..2137	-	351	WP_004197756.1	hypothetical protein	-
LZ608_RS29305 (LZ608_29305)	2203..2748	-	546	WP_023317533.1	hypothetical protein	-
LZ608_RS29310 (LZ608_29310)	2774..3100	-	327	WP_050516389.1	hypothetical protein	-
LZ608_RS29315 (LZ608_29315)	4256..4432	+	177	WP_004197757.1	hypothetical protein	-
LZ608_RS29320 (LZ608_29320)	4422..4766	+	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
LZ608_RS29325 (LZ608_29325)	4948..5193	-	246	WP_032433971.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T230756 WP_004197762.1 NZ_CP090437:1451-1717 [Klebsiella pneumoniae subsp. pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T230756 NZ_CP125351:c3042153-3041902 [Escherichia fergusonii]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT230756 WP_000879771.1 NZ_CP090437:1243-1467 [Klebsiella pneumoniae subsp. pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT230756 NZ_CP125351:c3041905-3041651 [Escherichia fergusonii]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: S1EJF5