Detailed information of TA system TA230755

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 3750..4330
• Replicon: plasmid pD
• Accession: NZ_CP090436
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KPN857

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: LZ608_RS29240
• Protein ID: WP_071177730.1
• Coordinates: 4016..4330 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: LZ608_RS29235
• Protein ID: WP_000093040.1
• Coordinates: 3750..4028 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ608_RS29215 (LZ608_29215)	191..1678	-	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
LZ608_RS29220 (LZ608_29220)	2323..2568	+	246	WP_032440458.1	hypothetical protein	-
LZ608_RS29225 (LZ608_29225)	2842..3213	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
LZ608_RS29230 (LZ608_29230)	3210..3575	+	366	WP_072354022.1	TonB family protein	-
LZ608_RS29235 (LZ608_29235)	3750..4028	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
LZ608_RS29240 (LZ608_29240)	4016..4330	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LZ608_RS29245 (LZ608_29245)	4494..4922	+	429	WP_001140599.1	hypothetical protein	-
LZ608_RS29250 (LZ608_29250)	4948..5127	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
LZ608_RS29255 (LZ608_29255)	5154..5684	-	531	WP_071177729.1	hypothetical protein	-
LZ608_RS29260 (LZ608_29260)	5691..6422	-	732	WP_071177728.1	MobC family replication-relaxation protein	-
LZ608_RS29265 (LZ608_29265)	6422..8386	-	1965	WP_071177727.1	TraM recognition domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11970	11970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T230755 WP_071177730.1 NZ_CP090436:4016-4330 [Klebsiella pneumoniae subsp. pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T230755 NZ_CP125351:c3041905-3041651 [Escherichia fergusonii]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT230755 WP_000093040.1 NZ_CP090436:3750-4028 [Klebsiella pneumoniae subsp. pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT230755 NZ_CP125351:c3042153-3041902 [Escherichia fergusonii]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1