Detailed information of TA system TA023075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 2468635..2469271
• Replicon: chromosome
• Accession: NC_011206
• Organism: Acidithiobacillus ferrooxidans ATCC 53993

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B7J980
• Locus tag: LFERR_RS12390
• Protein ID: WP_012537455.1
• Coordinates: 2468635..2469051 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B7J981
• Locus tag: LFERR_RS12395
• Protein ID: WP_009560865.1
• Coordinates: 2469038..2469271 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFERR_RS15110	2463815..2463967	-	153	WP_009566804.1	integrase core domain-containing protein	-
LFERR_RS14625	2463974..2464261	-	288	WP_012537449.1	DDE-type integrase/transposase/recombinase	-
LFERR_RS12370	2464624..2465550	-	927	WP_012537450.1	uracil-DNA glycosylase	-
LFERR_RS12375	2465717..2466787	+	1071	WP_012537451.1	linear amide C-N hydrolase	-
LFERR_RS15115	2467000..2467389	-	390	WP_111122584.1	type II toxin-antitoxin system PemK/MazF family toxin	-
LFERR_RS12380	2467380..2467631	-	252	WP_012537453.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
LFERR_RS15510	2467951..2468532	+	582	WP_146235847.1	hypothetical protein	-
LFERR_RS12390	2468635..2469051	-	417	WP_012537455.1	PIN domain-containing protein	Toxin
LFERR_RS12395	2469038..2469271	-	234	WP_009560865.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LFERR_RS12400	2469270..2469530	+	261	WP_012607558.1	hypothetical protein	-
LFERR_RS12405	2469627..2469977	+	351	WP_012537457.1	DUF4870 domain-containing protein	-
LFERR_RS12410	2470074..2471936	+	1863	WP_012537458.1	ATP-binding cassette domain-containing protein	-
LFERR_RS12415	2471933..2472256	-	324	WP_012537459.1	hypothetical protein	-
LFERR_RS12420	2472249..2472560	-	312	WP_009566855.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-119)

Antitoxin

• SpoVT-AbrB domain (5-37)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15358.75 Da
• Isoelectric Point: 5.1352

>T23075 WP_012537455.1 NC_011206:c2469051-2468635 [Acidithiobacillus ferrooxidans ATCC 53993]
MASGKAFFDTNVLLYLLSADTAKADVAEGIMVNGGVISAQVLNEFASVAQRKMGMSFLEIREVLTPIHRICDVAHLTLDT
HIHGLRIAERYKYNVWDAMIVASALLADCAVLYTEDMHHGQILEDRLQLINPFLRADE

Nucleotide

• Length: 417 bp

>T23075 NC_011206:c2469051-2468635 [Acidithiobacillus ferrooxidans ATCC 53993]
ATGGCGAGCGGTAAGGCGTTCTTCGACACGAACGTGCTGCTTTATCTGCTTTCGGCGGACACGGCCAAGGCAGATGTTGC
AGAAGGCATCATGGTGAATGGTGGTGTGATCAGTGCCCAGGTGTTGAACGAATTTGCTTCCGTTGCCCAGCGCAAGATGG
GCATGTCTTTTCTGGAAATCAGAGAGGTGCTGACCCCCATTCATCGAATTTGCGATGTCGCGCATCTTACCCTGGATACG
CACATTCATGGCCTCAGGATTGCGGAGCGATACAAATACAATGTTTGGGATGCAATGATTGTCGCATCGGCCCTGTTGGC
TGATTGCGCCGTTCTTTACACGGAGGATATGCACCACGGCCAGATCCTCGAAGACCGCTTGCAACTGATCAATCCATTTT
TGCGGGCCGACGAGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8919.15 Da
• Isoelectric Point: 8.7371

>AT23075 WP_009560865.1 NC_011206:c2469271-2469038 [Acidithiobacillus ferrooxidans ATCC 53993]
MQVSKWGNSLAVRIPTSVVEALGIKEGDDIEVEVVGERRFQIQRTPQAEELLARLRRYRGRLPADFRFDRLEAHGER

Nucleotide

• Length: 234 bp

>AT23075 NC_011206:c2469271-2469038 [Acidithiobacillus ferrooxidans ATCC 53993]
ATGCAGGTATCCAAGTGGGGCAACAGTCTGGCCGTCCGTATACCAACCTCCGTGGTAGAGGCGCTGGGTATCAAAGAGGG
TGACGACATTGAGGTTGAGGTCGTGGGCGAACGCCGTTTTCAGATTCAACGAACGCCTCAAGCGGAAGAGTTGTTGGCCA
GGTTAAGGCGTTATCGAGGGAGGCTCCCTGCCGATTTCAGGTTCGACCGGTTGGAAGCGCATGGCGAGCGGTAA

Structures

Toxin

• AlphaFold DB: B7J980

Antitoxin

• AlphaFold DB: B7J981