Detailed information of TA system TA230749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 117780..118450
• Replicon: plasmid pA
• Accession: NZ_CP090433
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KPN857

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: LZ608_RS27460
• Protein ID: WP_004213072.1
• Coordinates: 117780..118223 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: LZ608_RS27465
• Protein ID: WP_004213073.1
• Coordinates: 118220..118450 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ608_RS27425 (LZ608_27425)	113191..113466	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
LZ608_RS27430 (LZ608_27430)	113529..114020	+	492	WP_011251327.1	DM13 domain-containing protein	-
LZ608_RS27435 (LZ608_27435)	114069..114989	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
LZ608_RS27440 (LZ608_27440)	115080..115483	+	404	Protein_131	GAF domain-containing protein	-
LZ608_RS27445 (LZ608_27445)	116001..116636	-	636	Protein_132	mucoid phenotype regulator RmpA2	-
LZ608_RS27450 (LZ608_27450)	117053..117357	+	305	Protein_133	transposase	-
LZ608_RS27455 (LZ608_27455)	117380..117631	-	252	WP_186987481.1	hypothetical protein	-
LZ608_RS27460 (LZ608_27460)	117780..118223	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
LZ608_RS27465 (LZ608_27465)	118220..118450	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LZ608_RS27470 (LZ608_27470)	119058..120191	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
LZ608_RS27475 (LZ608_27475)	120207..120500	+	294	WP_004213076.1	hypothetical protein	-
LZ608_RS27480 (LZ608_27480)	120490..120696	-	207	WP_004213077.1	hypothetical protein	-
LZ608_RS27485 (LZ608_27485)	121048..121338	+	291	WP_004213078.1	hypothetical protein	-
LZ608_RS27490 (LZ608_27490)	121328..122227	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / iucA / iucB / iucC / iucD / iutA / rmpA2	1..192941	192941

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T230749 WP_004213072.1 NZ_CP090433:c118223-117780 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T230749 NZ_CP125351:792244-792558 [Escherichia fergusonii]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCATATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACACGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACTTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT230749 WP_004213073.1 NZ_CP090433:c118450-118220 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT230749 NZ_CP125351:792008-792241 [Escherichia fergusonii]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620