Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5357120..5357745 Replicon chromosome
Accession NZ_CP090432
Organism Klebsiella pneumoniae subsp. pneumoniae strain KPN857

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LZ608_RS26420 Protein ID WP_002882817.1
Coordinates 5357120..5357503 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LZ608_RS26425 Protein ID WP_004150355.1
Coordinates 5357503..5357745 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZ608_RS26405 (LZ608_26405) 5354486..5355388 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LZ608_RS26410 (LZ608_26410) 5355385..5356020 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LZ608_RS26415 (LZ608_26415) 5356017..5356946 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LZ608_RS26420 (LZ608_26420) 5357120..5357503 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LZ608_RS26425 (LZ608_26425) 5357503..5357745 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LZ608_RS26430 (LZ608_26430) 5357950..5358867 + 918 WP_002882812.1 alpha/beta hydrolase -
LZ608_RS26435 (LZ608_26435) 5358881..5359822 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LZ608_RS26440 (LZ608_26440) 5359867..5360304 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LZ608_RS26445 (LZ608_26445) 5360301..5361161 - 861 WP_002882807.1 virulence factor BrkB family protein -
LZ608_RS26450 (LZ608_26450) 5361155..5361754 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T230745 WP_002882817.1 NZ_CP090432:c5357503-5357120 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T230745 NZ_CP125350:c115778-115335 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT230745 WP_004150355.1 NZ_CP090432:c5357745-5357503 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT230745 NZ_CP125350:c116005-115775 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References