Detailed information of TA system TA230743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4875685..4876201
• Replicon: chromosome
• Accession: NZ_CP090432
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KPN857

Toxin (Protein)

• Gene name: relE
• Uniprot ID: J2XDK6
• Locus tag: LZ608_RS24105
• Protein ID: WP_002886902.1
• Coordinates: 4875685..4875969 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: R4Y888
• Locus tag: LZ608_RS24110
• Protein ID: WP_002886901.1
• Coordinates: 4875959..4876201 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ608_RS24080 (LZ608_24080)	4871169..4871432	-	264	WP_004152271.1	PTS sugar transporter subunit IIB	-
LZ608_RS24085 (LZ608_24085)	4871562..4871735	+	174	WP_002886906.1	hypothetical protein	-
LZ608_RS24090 (LZ608_24090)	4871738..4872481	+	744	WP_002886905.1	MurR/RpiR family transcriptional regulator	-
LZ608_RS24095 (LZ608_24095)	4872838..4874976	+	2139	WP_002886904.1	anaerobic ribonucleoside-triphosphate reductase	-
LZ608_RS24100 (LZ608_24100)	4875217..4875681	+	465	WP_002886903.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
LZ608_RS24105 (LZ608_24105)	4875685..4875969	-	285	WP_002886902.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LZ608_RS24110 (LZ608_24110)	4875959..4876201	-	243	WP_002886901.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LZ608_RS24115 (LZ608_24115)	4876279..4878189	-	1911	WP_004152270.1	PRD domain-containing protein	-
LZ608_RS24120 (LZ608_24120)	4878212..4879366	-	1155	WP_002886900.1	lactonase family protein	-
LZ608_RS24125 (LZ608_24125)	4879432..4880172	-	741	WP_002886899.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11125.95 Da
• Isoelectric Point: 10.3787

>T230743 WP_002886902.1 NZ_CP090432:c4875969-4875685 [Klebsiella pneumoniae subsp. pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESARLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Nucleotide

• Length: 285 bp

>T230743 NZ_CP125350:c33420-33109 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9045.55 Da
• Isoelectric Point: 6.3340

>AT230743 WP_002886901.1 NZ_CP090432:c4876201-4875959 [Klebsiella pneumoniae subsp. pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Nucleotide

• Length: 243 bp

>AT230743 NZ_CP125350:c33112-32693 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A0H3GMH2

Antitoxin

• AlphaFold DB: A0A0H3GLP0