Detailed information of TA system TA230740

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 3334246..3334877
• Replicon: chromosome
• Accession: NZ_CP090432
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KPN857

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1Q8YTV3
• Locus tag: LZ608_RS16705
• Protein ID: WP_012542177.1
• Coordinates: 3334701..3334877 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A3Q9U6R4
• Locus tag: LZ608_RS16700
• Protein ID: WP_017898984.1
• Coordinates: 3334246..3334653 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ608_RS16665 (LZ608_16665)	3329610..3330044	-	435	WP_012542168.1	phage terminase small subunit P27 family	-
LZ608_RS16670 (LZ608_16670)	3330292..3330723	-	432	WP_023279521.1	hypothetical protein	-
LZ608_RS16675 (LZ608_16675)	3330720..3331037	-	318	WP_023279522.1	hypothetical protein	-
LZ608_RS16680 (LZ608_16680)	3330989..3331351	-	363	WP_014228901.1	HNH endonuclease	-
LZ608_RS16685 (LZ608_16685)	3332476..3332826	-	351	WP_017898986.1	hypothetical protein	-
LZ608_RS16690 (LZ608_16690)	3332823..3333320	-	498	WP_023279523.1	lysozyme	-
LZ608_RS16695 (LZ608_16695)	3333320..3333535	-	216	WP_017880269.1	class II holin family protein	-
LZ608_RS16700 (LZ608_16700)	3334246..3334653	-	408	WP_017898984.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LZ608_RS16705 (LZ608_16705)	3334701..3334877	-	177	WP_012542177.1	type II toxin-antitoxin system HicA family toxin	Toxin
LZ608_RS16710 (LZ608_16710)	3335241..3337025	+	1785	WP_032415155.1	ATP-binding protein	-
LZ608_RS16715 (LZ608_16715)	3337045..3338079	+	1035	WP_219071718.1	DNA cytosine methyltransferase	-
LZ608_RS16720 (LZ608_16720)	3338104..3338445	-	342	WP_017898982.1	antiterminator Q family protein	-
LZ608_RS16725 (LZ608_16725)	3338458..3339489	-	1032	WP_071058942.1	DUF968 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3300777..3358288	57511

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-61)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6632.83 Da
• Isoelectric Point: 11.0174

>T230740 WP_012542177.1 NZ_CP090432:c3334877-3334701 [Klebsiella pneumoniae subsp. pneumoniae]
VKQSELRRWLAAQGAEFKDGTNHLKIYLNGKQTVMPRHPGKEIPEPLRKAILKQLGIK

Nucleotide

• Length: 177 bp

>T230740 NZ_CP125349:c4908533-4908249 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14959.96 Da
• Isoelectric Point: 4.4277

>AT230740 WP_017898984.1 NZ_CP090432:c3334653-3334246 [Klebsiella pneumoniae subsp. pneumoniae]
MRYPVIFEHDETGWAVFFPDIPEAMTGGETREEALEMAQDALVTAFDFYFDDRREIPAPSAEGDAFVEVPASVAAKVLLL
NRLVSTNTSNADLARLINTRPQEVQRIVSLGHSTKIDTIQKALSALGQKMEIVVH

Nucleotide

• Length: 408 bp

>AT230740 NZ_CP125349:c4908765-4908523 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A1Q8YTV3

Antitoxin

• AlphaFold DB: A0A3Q9U6R4