Detailed information of TA system TA023073

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1414294..1414968
• Replicon: chromosome
• Accession: NC_011206
• Organism: Acidithiobacillus ferrooxidans ATCC 53993

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B7JBN6
• Locus tag: LFERR_RS07210
• Protein ID: WP_009564531.1
• Coordinates: 1414549..1414968 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B7JBN5
• Locus tag: LFERR_RS07205
• Protein ID: WP_012536752.1
• Coordinates: 1414294..1414548 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFERR_RS07190	1409969..1410973	-	1005	WP_012536749.1	glycosyltransferase family 2 protein	-
LFERR_RS07195	1411112..1412572	+	1461	WP_012536750.1	IMP dehydrogenase	-
LFERR_RS07200	1412576..1414120	+	1545	WP_012536751.1	glutamine-hydrolyzing GMP synthase	-
LFERR_RS07205	1414294..1414548	+	255	WP_012536752.1	plasmid stabilization protein	Antitoxin
LFERR_RS07210	1414549..1414968	+	420	WP_009564531.1	type II toxin-antitoxin system VapC family toxin	Toxin
LFERR_RS07215	1414986..1415198	+	213	WP_009564530.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
LFERR_RS14930	1415198..1415320	+	123	Protein_1438	VapC toxin family PIN domain ribonuclease	-
LFERR_RS07220	1416033..1417652	+	1620	WP_012536754.1	APC family permease	-
LFERR_RS07225	1417743..1418942	+	1200	WP_012536755.1	glutamate 5-kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14968.11 Da
• Isoelectric Point: 4.7051

>T23073 WP_009564531.1 NC_011206:1414549-1414968 [Acidithiobacillus ferrooxidans ATCC 53993]
MIVLDTNVVSEAMKPEPDAAVRAWLNEQAAETLYLSSVTLAELLFGIRALPAGKCKNMLDRALTELLELFKDRVLPFDTD
AARHYADLAVTAKNGGRGFPTPDGYIAAVAASRGFIVASRDTSPYEAAGVTVINPWKDA

Nucleotide

• Length: 420 bp

>T23073 NC_011206:1414549-1414968 [Acidithiobacillus ferrooxidans ATCC 53993]
ATGATCGTCCTGGATACCAACGTCGTTTCCGAGGCGATGAAGCCGGAGCCAGACGCGGCCGTGAGGGCTTGGCTGAACGA
ACAGGCGGCGGAAACCTTGTATCTGTCGAGCGTCACGCTGGCCGAGTTGCTGTTCGGCATCCGGGCGCTGCCGGCGGGCA
AGTGCAAGAACATGCTCGACCGCGCCCTGACCGAACTGCTGGAGCTGTTCAAGGATCGGGTGCTGCCCTTCGATACGGAT
GCAGCGCGCCACTATGCCGACCTGGCCGTGACGGCCAAGAACGGCGGGCGCGGCTTCCCGACGCCAGACGGCTACATCGC
GGCTGTTGCCGCATCGCGGGGCTTCATCGTGGCATCACGCGACACGTCGCCCTATGAAGCGGCCGGCGTGACCGTCATCA
ATCCCTGGAAGGATGCATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9225.67 Da
• Isoelectric Point: 10.7239

>AT23073 WP_012536752.1 NC_011206:1414294-1414548 [Acidithiobacillus ferrooxidans ATCC 53993]
MAILTVRNVPDDVHRALRVRAARHGRSTEAEVREILAAAVKPSSRVCMGEALAAIGRKIGLTNEDFAVFESVRDKTPAKP
LRFD

Nucleotide

• Length: 255 bp

>AT23073 NC_011206:1414294-1414548 [Acidithiobacillus ferrooxidans ATCC 53993]
ATGGCAATCCTGACTGTAAGAAATGTGCCGGATGACGTGCATCGCGCTTTGCGCGTGCGGGCGGCCCGGCACGGTCGCAG
CACGGAAGCGGAGGTGCGCGAGATTCTGGCGGCTGCGGTCAAACCTTCGAGCCGCGTGTGCATGGGGGAAGCCCTGGCCG
CCATCGGCCGCAAGATCGGTCTGACCAATGAAGATTTCGCCGTTTTCGAGAGTGTGCGCGACAAGACGCCGGCCAAACCG
TTGCGCTTCGACTGA

Structures

Toxin

• AlphaFold DB: B7JBN6

Antitoxin

• AlphaFold DB: B7JBN5