Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 3942411..3943047 Replicon chromosome
Accession NZ_CP090431
Organism Priestia flexa strain DMP08

Toxin (Protein)


Gene name mazF Uniprot ID A0A0V8JQ27
Locus tag LZP85_RS20370 Protein ID WP_025910606.1
Coordinates 3942697..3943047 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1N6ZQI9
Locus tag LZP85_RS20365 Protein ID WP_025910605.1
Coordinates 3942411..3942692 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZP85_RS20340 (LZP85_20340) 3937687..3938655 + 969 WP_119542900.1 UV DNA damage repair endonuclease UvsE -
LZP85_RS20345 (LZP85_20345) 3938691..3939284 - 594 WP_224840390.1 rhomboid family intramembrane serine protease -
LZP85_RS20350 (LZP85_20350) 3939376..3939744 + 369 WP_025910601.1 holo-ACP synthase -
LZP85_RS20355 (LZP85_20355) 3939858..3940874 + 1017 WP_025910603.1 outer membrane lipoprotein carrier protein LolA -
LZP85_RS20360 (LZP85_20360) 3941063..3942235 + 1173 WP_119542899.1 alanine racemase -
LZP85_RS20365 (LZP85_20365) 3942411..3942692 + 282 WP_025910605.1 hypothetical protein Antitoxin
LZP85_RS20370 (LZP85_20370) 3942697..3943047 + 351 WP_025910606.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LZP85_RS20375 (LZP85_20375) 3943221..3944057 + 837 WP_119542898.1 RsbT co-antagonist protein RsbRA -
LZP85_RS20380 (LZP85_20380) 3944054..3944416 + 363 WP_035321022.1 STAS domain-containing protein -
LZP85_RS20385 (LZP85_20385) 3944420..3944821 + 402 WP_025910609.1 anti-sigma regulatory factor -
LZP85_RS20390 (LZP85_20390) 3944833..3945846 + 1014 WP_119542897.1 PP2C family protein-serine/threonine phosphatase -
LZP85_RS20395 (LZP85_20395) 3945909..3946241 + 333 WP_025910611.1 anti-sigma factor antagonist -
LZP85_RS20400 (LZP85_20400) 3946238..3946723 + 486 WP_061785866.1 anti-sigma B factor RsbW -
LZP85_RS20405 (LZP85_20405) 3946689..3947483 + 795 WP_025910613.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.94 Da        Isoelectric Point: 5.1442

>T230726 WP_025910606.1 NZ_CP090431:3942697-3943047 [Priestia flexa]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDDALQISVGLIDF

Download         Length: 351 bp

>T230726 NZ_CP125344:130425-130868 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10654.20 Da        Isoelectric Point: 9.5610

>AT230726 WP_025910605.1 NZ_CP090431:3942411-3942692 [Priestia flexa]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSEFIYQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAES
EANHTVDRLVSGG

Download         Length: 282 bp

>AT230726 NZ_CP125344:130198-130428 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V8JQ27

Antitoxin

Source ID Structure
AlphaFold DB A0A1N6ZQI9

References