Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazF-pemI/MazF(toxin) |
| Location | 3942411..3943047 | Replicon | chromosome |
| Accession | NZ_CP090431 | ||
| Organism | Priestia flexa strain DMP08 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | A0A0V8JQ27 |
| Locus tag | LZP85_RS20370 | Protein ID | WP_025910606.1 |
| Coordinates | 3942697..3943047 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | pemI | Uniprot ID | A0A1N6ZQI9 |
| Locus tag | LZP85_RS20365 | Protein ID | WP_025910605.1 |
| Coordinates | 3942411..3942692 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LZP85_RS20340 (LZP85_20340) | 3937687..3938655 | + | 969 | WP_119542900.1 | UV DNA damage repair endonuclease UvsE | - |
| LZP85_RS20345 (LZP85_20345) | 3938691..3939284 | - | 594 | WP_224840390.1 | rhomboid family intramembrane serine protease | - |
| LZP85_RS20350 (LZP85_20350) | 3939376..3939744 | + | 369 | WP_025910601.1 | holo-ACP synthase | - |
| LZP85_RS20355 (LZP85_20355) | 3939858..3940874 | + | 1017 | WP_025910603.1 | outer membrane lipoprotein carrier protein LolA | - |
| LZP85_RS20360 (LZP85_20360) | 3941063..3942235 | + | 1173 | WP_119542899.1 | alanine racemase | - |
| LZP85_RS20365 (LZP85_20365) | 3942411..3942692 | + | 282 | WP_025910605.1 | hypothetical protein | Antitoxin |
| LZP85_RS20370 (LZP85_20370) | 3942697..3943047 | + | 351 | WP_025910606.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| LZP85_RS20375 (LZP85_20375) | 3943221..3944057 | + | 837 | WP_119542898.1 | RsbT co-antagonist protein RsbRA | - |
| LZP85_RS20380 (LZP85_20380) | 3944054..3944416 | + | 363 | WP_035321022.1 | STAS domain-containing protein | - |
| LZP85_RS20385 (LZP85_20385) | 3944420..3944821 | + | 402 | WP_025910609.1 | anti-sigma regulatory factor | - |
| LZP85_RS20390 (LZP85_20390) | 3944833..3945846 | + | 1014 | WP_119542897.1 | PP2C family protein-serine/threonine phosphatase | - |
| LZP85_RS20395 (LZP85_20395) | 3945909..3946241 | + | 333 | WP_025910611.1 | anti-sigma factor antagonist | - |
| LZP85_RS20400 (LZP85_20400) | 3946238..3946723 | + | 486 | WP_061785866.1 | anti-sigma B factor RsbW | - |
| LZP85_RS20405 (LZP85_20405) | 3946689..3947483 | + | 795 | WP_025910613.1 | RNA polymerase sigma factor SigB | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12948.94 Da Isoelectric Point: 5.1442
>T230726 WP_025910606.1 NZ_CP090431:3942697-3943047 [Priestia flexa]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDDALQISVGLIDF
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDDALQISVGLIDF
Download Length: 351 bp
>T230726 NZ_CP125344:130425-130868 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA
Antitoxin
Download Length: 94 a.a. Molecular weight: 10654.20 Da Isoelectric Point: 9.5610
>AT230726 WP_025910605.1 NZ_CP090431:3942411-3942692 [Priestia flexa]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSEFIYQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAES
EANHTVDRLVSGG
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSEFIYQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAES
EANHTVDRLVSGG
Download Length: 282 bp
>AT230726 NZ_CP125344:130198-130428 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0V8JQ27 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1N6ZQI9 |