Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4648980..4649790 Replicon chromosome
Accession NZ_CP090429
Organism Klebsiella pneumoniae strain DY1928

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LYZ32_RS22955 Protein ID WP_004178461.1
Coordinates 4648980..4649513 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A483I2M4
Locus tag LYZ32_RS22960 Protein ID WP_004222378.1
Coordinates 4649524..4649790 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LYZ32_RS22950 (4647811) 4647811..4648932 + 1122 WP_117116885.1 cupin domain-containing protein -
LYZ32_RS22955 (4648980) 4648980..4649513 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LYZ32_RS22960 (4649524) 4649524..4649790 - 267 WP_004222378.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LYZ32_RS22965 (4649893) 4649893..4651326 - 1434 WP_004222374.1 Cu(+)/Ag(+) sensor histidine kinase -
LYZ32_RS22970 (4651316) 4651316..4651999 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LYZ32_RS22975 (4652171) 4652171..4653556 + 1386 WP_004222371.1 efflux transporter outer membrane subunit -
LYZ32_RS22980 (4653574) 4653574..4653918 + 345 WP_004222368.1 cation efflux system protein CusF -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(91-151)

Antitoxin

(8-86)


Sequences


Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T230721 WP_004178461.1 NZ_CP090429:c4649513-4648980 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T230721 NZ_CP125343:4192194-4192412 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9969.44 Da        Isoelectric Point: 10.1757

>AT230721 WP_004222378.1 NZ_CP090429:c4649790-4649524 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLSEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT230721 NZ_CP125343:4191793-4192167 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A060VJ83


Antitoxin

Source ID Structure
AlphaFold DB A0A483I2M4

References