Detailed information of TA system TA023072

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapB(antitoxin)
• Location: 1256907..1257475
• Replicon: chromosome
• Accession: NC_011206
• Organism: Acidithiobacillus ferrooxidans ATCC 53993

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A5J6VSB9
• Locus tag: LFERR_RS06430
• Protein ID: WP_012536619.1
• Coordinates: 1256907..1257281 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2Z6IL32
• Locus tag: LFERR_RS14885
• Protein ID: WP_012536620.1
• Coordinates: 1257278..1257475 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFERR_RS06415	1253013..1253710	-	698	Protein_1274	putative DNA binding domain-containing protein	-
LFERR_RS06420	1253707..1256505	-	2799	WP_012536617.1	class I SAM-dependent DNA methyltransferase	-
LFERR_RS15290	1256623..1256766	-	144	Protein_1276	SAM-dependent DNA methyltransferase	-
LFERR_RS06430	1256907..1257281	-	375	WP_012536619.1	type II toxin-antitoxin system VapC family toxin	Toxin
LFERR_RS14885	1257278..1257475	-	198	WP_012536620.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LFERR_RS15295	1257821..1257982	-	162	WP_012536621.1	hypothetical protein	-
LFERR_RS06435	1258332..1259006	+	675	WP_012536622.1	hypothetical protein	-
LFERR_RS06440	1259003..1259326	+	324	WP_012536623.1	integration host factor subunit alpha	-
LFERR_RS06445	1259393..1259668	+	276	WP_012536624.1	HU family DNA-binding protein	-
LFERR_RS06450	1259680..1260033	+	354	WP_009560827.1	hypothetical protein	-
LFERR_RS14890	1260042..1260263	+	222	WP_009560828.1	HU family DNA-binding protein	-
LFERR_RS06455	1260866..1261000	-	135	Protein_1285	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
LFERR_RS06460	1260987..1261256	-	270	WP_012536625.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
LFERR_RS06465	1261281..1262177	-	897	WP_012536626.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1234011..1392831	158820

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-109)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-46)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13683.71 Da
• Isoelectric Point: 6.3157

>T23072 WP_012536619.1 NC_011206:c1257281-1256907 [Acidithiobacillus ferrooxidans ATCC 53993]
MILVDTSVWIDHFRSGDAHLSTLLDHAMVQTHDFIIGEIACGNLKNRQQTLYLLSGLPRCTATSPEETLFFIGRHSLMGR
GIGYIDASILASAMLVQANLWSRDRRLMALATELGCAYSPTSSE

Nucleotide

• Length: 375 bp

>T23072 NC_011206:c1257281-1256907 [Acidithiobacillus ferrooxidans ATCC 53993]
TTGATTCTGGTCGATACCTCCGTCTGGATCGACCATTTCCGCTCTGGAGATGCACACCTCTCTACTCTGCTTGATCATGC
CATGGTGCAAACGCACGATTTCATCATCGGAGAAATCGCTTGCGGGAATCTCAAAAATCGGCAGCAGACCTTATATCTGC
TCTCAGGCTTACCCCGATGCACCGCCACCTCTCCAGAAGAAACCCTGTTTTTTATTGGGCGTCATAGCCTGATGGGACGC
GGTATAGGCTATATTGACGCGAGCATCCTAGCCTCCGCCATGCTCGTGCAGGCCAATTTATGGTCCCGCGACAGACGTCT
CATGGCTCTGGCTACCGAACTTGGCTGCGCGTACTCGCCGACGTCATCCGAATAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7266.37 Da
• Isoelectric Point: 9.4896

>AT23072 WP_012536620.1 NC_011206:c1257475-1257278 [Acidithiobacillus ferrooxidans ATCC 53993]
MRTTINLDDELLAQAKTLCGVEDRGALLKEALRALIERESARRLARLGGTEPQLQNIPRRREPAA

Nucleotide

• Length: 198 bp

>AT23072 NC_011206:c1257475-1257278 [Acidithiobacillus ferrooxidans ATCC 53993]
ATGCGAACGACAATCAACCTGGATGATGAGCTCCTGGCCCAAGCCAAAACGCTCTGTGGCGTAGAAGACCGTGGCGCCCT
CTTAAAAGAGGCCCTGCGTGCTTTGATTGAACGGGAAAGTGCCCGTCGCCTCGCTCGCTTGGGTGGAACAGAACCGCAAT
TACAGAACATTCCTCGCAGACGAGAGCCTGCCGCTTGA

Structures

Toxin

• AlphaFold DB: A0A5J6VSB9

Antitoxin

• AlphaFold DB: A0A2Z6IL32