Detailed information of TA system TA023071

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 1110001..1110764
• Replicon: chromosome
• Accession: NC_011206
• Organism: Acidithiobacillus ferrooxidans ATCC 53993

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: B7J9L5
• Locus tag: LFERR_RS05680
• Protein ID: WP_009565062.1
• Coordinates: 1110001..1110495 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: B7J9L6
• Locus tag: LFERR_RS05685
• Protein ID: WP_012536505.1
• Coordinates: 1110492..1110764 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFERR_RS05655	1105778..1106548	+	771	WP_012536502.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
LFERR_RS05660	1106541..1107383	+	843	WP_012536503.1	Hsp33 family molecular chaperone HslO	-
LFERR_RS05665	1108101..1108607	-	507	WP_009569577.1	type II toxin-antitoxin system YhaV family toxin	-
LFERR_RS05670	1108604..1108933	-	330	WP_009569578.1	type II toxin-antitoxin system PrlF family antitoxin	-
LFERR_RS15605	1109220..1109363	+	144	WP_009569579.1	hypothetical protein	-
LFERR_RS05675	1109390..1109656	-	267	WP_012536504.1	type I restriction-modification system subunit M N-terminal domain-containing protein	-
LFERR_RS05680	1110001..1110495	-	495	WP_009565062.1	GNAT family N-acetyltransferase	Toxin
LFERR_RS05685	1110492..1110764	-	273	WP_012536505.1	DUF1778 domain-containing protein	Antitoxin
LFERR_RS05690	1110845..1111057	-	213	WP_009562915.1	hypothetical protein	-
LFERR_RS05695	1111225..1111441	-	217	Protein_1128	hypothetical protein	-
LFERR_RS05700	1111841..1112662	-	822	WP_012536506.1	UDP-2,3-diacylglucosamine diphosphatase	-
LFERR_RS05705	1112903..1113148	+	246	WP_009568482.1	DUF2934 domain-containing protein	-
LFERR_RS05710	1113343..1114479	+	1137	WP_012536507.1	glycosyltransferase family 1 protein	-
LFERR_RS05715	1114513..1115727	+	1215	WP_012536508.1	glycosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (4-87)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18013.83 Da
• Isoelectric Point: 10.4415

>T23071 WP_009565062.1 NC_011206:c1110495-1110001 [Acidithiobacillus ferrooxidans ATCC 53993]
MSLKLSAPQPLAAVHLLGDFESGEPALDEWLKRRAMNNQLTGASRTFVVVDEENRVRGFYAMAAGAVAHQVATSSVRRNM
PDPVPVMVLGRLAVDSRAQGIKLGAAMLQDAVKRAITVSQNTGVRALLVHALHERAKQFYEHYGFQESPQHPMTLMLRLN
SIKA

Nucleotide

• Length: 495 bp

>T23071 NC_011206:c1110495-1110001 [Acidithiobacillus ferrooxidans ATCC 53993]
ATGAGTTTGAAGCTATCCGCGCCGCAGCCACTTGCGGCCGTGCACCTGTTGGGCGACTTCGAGAGCGGTGAGCCTGCACT
GGATGAGTGGTTAAAACGCCGGGCGATGAACAACCAGCTCACCGGGGCCAGTCGAACCTTTGTGGTCGTTGATGAAGAAA
ATCGGGTGCGCGGCTTCTACGCCATGGCGGCTGGCGCAGTCGCACATCAAGTGGCGACTAGCTCGGTTCGTCGCAATATG
CCCGATCCGGTTCCCGTCATGGTGCTCGGTAGGTTGGCGGTGGATAGTCGAGCGCAAGGCATCAAGCTGGGGGCGGCCAT
GCTGCAGGACGCGGTGAAACGTGCCATCACCGTATCGCAGAACACGGGGGTTCGTGCACTGCTGGTTCATGCGCTCCACG
AACGCGCCAAACAGTTCTATGAGCACTACGGCTTTCAGGAGTCACCACAACATCCGATGACGCTGATGTTGCGCCTGAAT
AGCATCAAGGCTTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10071.52 Da
• Isoelectric Point: 5.0914

>AT23071 WP_012536505.1 NC_011206:c1110764-1110492 [Acidithiobacillus ferrooxidans ATCC 53993]
MRAAAINLRALPEQRDLIDHAANLLGKNRSDFMLEAACERAQSVLLDQVFFGLDAEKFRQFTAMLDAPPRGNEGLERLMA
VKAPWESNQA

Nucleotide

• Length: 273 bp

>AT23071 NC_011206:c1110764-1110492 [Acidithiobacillus ferrooxidans ATCC 53993]
ATGCGTGCCGCTGCCATCAATTTACGAGCCTTGCCGGAGCAGCGTGATCTCATTGATCACGCCGCCAACCTGTTAGGCAA
AAACCGTTCGGACTTCATGCTGGAAGCCGCCTGCGAGCGTGCGCAATCGGTACTGCTCGATCAGGTGTTTTTTGGGCTGG
ATGCGGAAAAGTTCCGGCAGTTCACCGCCATGCTCGACGCACCGCCTCGTGGCAACGAAGGTCTTGAGCGTCTGATGGCA
GTCAAGGCGCCATGGGAAAGCAATCAAGCATGA

Structures

Toxin

• AlphaFold DB: B7J9L5

Antitoxin

• AlphaFold DB: B7J9L6