Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2241336..2241850 Replicon chromosome
Accession NZ_CP090407
Organism Staphylococcus aureus strain GD4SA159-1

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag LZ183_RS11015 Protein ID WP_000581792.1
Coordinates 2241336..2241545 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag LZ183_RS11020 Protein ID WP_000138932.1
Coordinates 2241557..2241850 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZ183_RS10995 (LZ183_10975) 2236847..2238202 - 1356 WP_000611457.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
LZ183_RS11000 (LZ183_10980) 2238217..2239287 - 1071 WP_000159641.1 D-alanine--D-alanine ligase -
LZ183_RS11005 (LZ183_10985) 2239605..2240807 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
LZ183_RS11010 (LZ183_10990) 2241071..2241208 - 138 WP_000828354.1 Lmo0850 family protein -
LZ183_RS11015 (LZ183_10995) 2241336..2241545 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
LZ183_RS11020 (LZ183_11000) 2241557..2241850 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
LZ183_RS11025 (LZ183_11005) 2242016..2243500 + 1485 WP_000571550.1 cardiolipin synthase -
LZ183_RS11030 (LZ183_11010) 2243528..2244175 + 648 WP_001187614.1 HD domain-containing protein -
LZ183_RS11035 (LZ183_11015) 2244732..2244794 + 63 Protein_2133 hypothetical protein -
LZ183_RS11040 (LZ183_11020) 2244772..2246087 - 1316 Protein_2134 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T230695 WP_000581792.1 NZ_CP090407:c2241545-2241336 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T230695 NZ_CP125334:737375-737860 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT230695 WP_000138932.1 NZ_CP090407:c2241850-2241557 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT230695 NZ_CP125334:737085-737378 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References