Detailed information of TA system TA230694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2221599..2222128
• Replicon: chromosome
• Accession: NZ_CP090407
• Organism: Staphylococcus aureus strain GD4SA159-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LZ183_RS10925
• Protein ID: WP_000621175.1
• Coordinates: 2221599..2221961 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: LZ183_RS10930
• Protein ID: WP_000948331.1
• Coordinates: 2221958..2222128 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ183_RS10905 (LZ183_10885)	2218577..2219347	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
LZ183_RS10910 (LZ183_10890)	2219322..2219801	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
LZ183_RS10915 (LZ183_10895)	2219803..2220129	-	327	WP_001052491.1	anti-sigma factor antagonist	-
LZ183_RS10920 (LZ183_10900)	2220248..2221249	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
LZ183_RS10925 (LZ183_10905)	2221599..2221961	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LZ183_RS10930 (LZ183_10910)	2221958..2222128	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
LZ183_RS10935 (LZ183_10915)	2222213..2223361	-	1149	WP_001281139.1	alanine racemase	-
LZ183_RS10940 (LZ183_10920)	2223427..2223786	-	360	WP_000581197.1	holo-ACP synthase	-
LZ183_RS10945 (LZ183_10925)	2223790..2224281	-	492	WP_001286980.1	PH domain-containing protein	-
LZ183_RS10950 (LZ183_10930)	2224274..2225851	-	1578	WP_001294650.1	PH domain-containing protein	-
LZ183_RS10955 (LZ183_10935)	2225844..2226323	-	480	WP_001287081.1	hypothetical protein	-
LZ183_RS10960 (LZ183_10940)	2226532..2227092	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T230694 WP_000621175.1 NZ_CP090407:c2221961-2221599 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T230694 NZ_CP125334:348551-348898 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT230694 WP_000948331.1 NZ_CP090407:c2222128-2221958 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT230694 NZ_CP125334:348898-349197 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1