Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 44459..44952 Replicon plasmid pPIB-3
Accession NZ_CP090405
Organism Shigella sp. PIB

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag LXH19_RS23705 Protein ID WP_001230709.1
Coordinates 44459..44713 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag LXH19_RS23710 Protein ID WP_000151456.1
Coordinates 44710..44952 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXH19_RS23665 (LXH19_23660) 39970..40122 + 153 Protein_49 FlmC family protein -
LXH19_RS23670 (LXH19_23665) 40070..40189 + 120 WP_228932012.1 Hok/Gef family protein -
LXH19_RS23675 (LXH19_23670) 40339..40935 + 597 WP_000125165.1 ProQ/FINO family protein -
LXH19_RS23680 (LXH19_23675) 40953..41237 - 285 WP_001162371.1 hypothetical protein -
LXH19_RS23685 (LXH19_23680) 41316..41981 - 666 WP_000532148.1 division plane positioning ATPase MipZ -
LXH19_RS23690 (LXH19_23685) 42571..42900 + 330 WP_001542022.1 hypothetical protein -
LXH19_RS23695 (LXH19_23690) 43301..43816 + 516 WP_001025393.1 J domain-containing protein -
LXH19_RS23700 (LXH19_23695) 43920..44462 + 543 WP_001083904.1 hypothetical protein -
LXH19_RS23705 (LXH19_23700) 44459..44713 + 255 WP_001230709.1 hypothetical protein Toxin
LXH19_RS23710 (LXH19_23705) 44710..44952 + 243 WP_000151456.1 hypothetical protein Antitoxin
LXH19_RS23715 (LXH19_23710) 45173..45391 + 219 WP_154101533.1 hypothetical protein -
LXH19_RS23720 (LXH19_23715) 45523..45816 + 294 WP_000673428.1 hypothetical protein -
LXH19_RS23725 (LXH19_23720) 46310..46558 + 249 WP_000513532.1 type II toxin-antitoxin system HicA family toxin -
LXH19_RS23730 (LXH19_23725) 46555..46902 + 348 WP_000681609.1 type II toxin-antitoxin system HicB family antitoxin -
LXH19_RS23735 (LXH19_23730) 46990..47433 - 444 WP_023223351.1 hypothetical protein -
LXH19_RS23740 (LXH19_23735) 47674..47973 + 300 WP_034169410.1 type II toxin-antitoxin system HicB family antitoxin -
LXH19_RS23745 (LXH19_23740) 48233..48559 - 327 WP_000206697.1 hypothetical protein -
LXH19_RS23750 (LXH19_23745) 48561..48899 - 339 WP_029797038.1 hypothetical protein -
LXH19_RS23755 (LXH19_23750) 48879..49001 - 123 Protein_67 hypothetical protein -
LXH19_RS23760 (LXH19_23755) 49021..49728 - 708 WP_000108794.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-1.1 - 1..63625 63625


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T230685 WP_001230709.1 NZ_CP090405:44459-44713 [Shigella sp. PIB]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T230685 NZ_CP125310:c5074102-5073728 [Citrobacter freundii]
ATGGTCAAAGGATCCGCGCTTTTTGATACCAACATCCTCATCGATCTGTTTAGTGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAATGCAATCAGCCTCATTACATGGATGGAGGTGATGGTGGGTGCAAAAAAATACCATCAGG
AAGCCCGTACTCGTGTTGCGCTAGGTGCTTTTAACGTGATCGGCGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGACAGGAATATGGGATGAAATTGCCGGATGCCATTATTTTGGCAACAGCACAGATTCATCGTCTTACGCTGGTTACGCG
AAATACTAAGGATTTTGCAGGCCTATCCGGTGTGGTCACACCGTATACATTATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT230685 WP_000151456.1 NZ_CP090405:44710-44952 [Shigella sp. PIB]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT230685 NZ_CP125310:c5074344-5074102 [Citrobacter freundii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAAGTGATTAAACGTCTTGATGATCTCAAGGT
GCAGCGCAACCTGCCGCGAGCAGAACTTTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAGGATCAGGCTGAAACCA
CAATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTCCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References