Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 15747..16294 Replicon plasmid pPIB-2
Accession NZ_CP090404
Organism Shigella sp. PIB

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag LXH19_RS23025 Protein ID WP_001384452.1
Coordinates 16016..16294 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID K4JYR0
Locus tag LXH19_RS23020 Protein ID WP_000079928.1
Coordinates 15747..16016 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXH19_RS22990 (LXH19_22985) 11088..11330 - 243 WP_021515452.1 PilI type IV pilus biogenesis protein -
LXH19_RS22995 (LXH19_22990) 11411..11554 - 144 WP_000176500.1 hypothetical protein -
LXH19_RS23000 (LXH19_22995) 11821..12483 - 663 WP_005082527.1 hypothetical protein -
LXH19_RS23005 (LXH19_23000) 12623..13129 - 507 WP_005082529.1 transcription termination/antitermination NusG family protein -
LXH19_RS23010 (LXH19_23005) 13967..14839 + 873 WP_000729438.1 incFII family plasmid replication initiator RepA -
LXH19_RS23015 (LXH19_23010) 15534..15692 + 159 WP_032151498.1 hypothetical protein -
LXH19_RS23020 (LXH19_23015) 15747..16016 + 270 WP_000079928.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
LXH19_RS23025 (LXH19_23020) 16016..16294 + 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
LXH19_RS23030 (LXH19_23025) 16808..17425 - 618 WP_025750220.1 ProQ/FINO family protein -
LXH19_RS23035 (LXH19_23030) 17734..18430 - 697 Protein_21 IS1 family transposase -
LXH19_RS23040 (LXH19_23035) 18664..19983 - 1320 WP_160470889.1 cation:dicarboxylase symporter family transporter -
LXH19_RS23045 (LXH19_23040) 20195..20891 + 697 Protein_23 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-14 - 1..87994 87994


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10744.34 Da        Isoelectric Point: 8.4615

>T230683 WP_001384452.1 NZ_CP090404:16016-16294 [Shigella sp. PIB]
IEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T230683 NZ_CP125310:c4923768-4923400 [Citrobacter freundii]
ATGACGCTACAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTGACGCCTGGTGTCGCAGG
TATGCCTGACCCCGGTCGTGCGGAAGCGCTGATGTATCGGGTATTGAATCAAATCGAATATGAAGGTGTGACTGACGTGT
GGCTACTGGCGGCAATGCATTTGTTAGCCATTTCCCGTGGGCATATCTTTAATGATGGTAACAAGCGTACTGCGTTATTT
ATTACGTTGCTGTTTTTAAAACGTAATGGGATCGTATTATCTGCAAACCCTGATTTTGTCGCCATGACGGTTGAAGCGGC
AGCAGGACAACTCACGTTAGAACAAATTGCGCATCGTCTGCGCCATTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10175.50 Da        Isoelectric Point: 7.3210

>AT230683 WP_000079928.1 NZ_CP090404:15747-16016 [Shigella sp. PIB]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT230683 NZ_CP125310:c4923986-4923765 [Citrobacter freundii]
ATGCGCACTATGAACTATAGTGAAGCTCGACAAAACCTGGCTGAAGTGCTGGAGAGCGCTGTCACTGGTGTGCCTGTTAC
CATTACGCGCCGTGGTCATAAACCTGCGGTAATCATCAGCGCGGAAGAGTTTGAACGCTATCAGGCCGCCAGACTTGATG
ATGAATTTGCCGACATAATGATGATTCATGGTAATGAAATCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A5W2J4D0

References