Detailed information of TA system TA230682

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 79818..80365
• Replicon: plasmid pPIB-1
• Accession: NZ_CP090403
• Organism: Shigella sp. PIB

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: LXH19_RS22815
• Protein ID: WP_244442846.1
• Coordinates: 79818..80096 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: LXH19_RS22820
• Protein ID: WP_000079941.1
• Coordinates: 80096..80365 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXH19_RS22785 (75207)	75207..76091	-	885	Protein_84	hypothetical protein	-
LXH19_RS22790 (76445)	76445..77047	+	603	WP_000517695.1	ProQ/FINO family protein	-
LXH19_RS22795 (77103)	77103..77597	+	495	WP_001459505.1	hypothetical protein	-
LXH19_RS22800 (77597)	77597..77863	+	267	WP_000955263.1	hypothetical protein	-
LXH19_RS22805 (78340)	78340..78813	-	474	WP_001334658.1	hypothetical protein	-
LXH19_RS22810 (78930)	78930..79778	-	849	WP_001057992.1	3'-5' exonuclease	-
LXH19_RS22815 (79818)	79818..80096	-	279	WP_244442846.1	type II toxin-antitoxin system toxin YacB	Toxin
LXH19_RS22820 (80096)	80096..80365	-	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
LXH19_RS22825 (80420)	80420..80578	-	159	WP_000841008.1	hypothetical protein	-
LXH19_RS22830 (81274)	81274..82305	-	1032	WP_000907873.1	plasmid replication initiator RepA	-
LXH19_RS22835 (83311)	83311..83541	+	231	WP_228772074.1	ash family protein	-
LXH19_RS22840 (83459)	83459..83746	+	288	WP_000074849.1	conjugal transfer protein TraA	-
LXH19_RS22845 (84188)	84188..84721	+	534	WP_001303319.1	transcription termination/antitermination NusG family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-2	-	1..98997	98997
	-	flank	IS/Tn	blaCMY-2	-	72293..75024	2731

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10761.43 Da
• Isoelectric Point: 9.7617

>T230682 WP_244442846.1 NZ_CP090403:c80096-79818 [Shigella sp. PIB]
MEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T230682 NZ_CP125310:4865743-4866060 [Citrobacter freundii]
ATGTTCACATTTATTGAGCTACAAGGATTTAGTAAACGCCGTCCGTTACTTTTGCCTGACGATGAGTTTCGCGCTTTTCA
AGAGGCGCTTATAGAGAACCCTGAGGCTGGAGATACTATCGCTGGTACAGGTGGATTCAGAAAAATTCGCTGGAGTCGCT
CTGGTATGGGCAAACGCAGCGGTATTAGGGTTATCTATTACAACGTGACGCGCAAAGGTCGAATCTATCTCGCTTTGCTC
TATCCTAAAAATGAACAGGATGATTTAACCGAAGAGCAAAAAAGAGTTTTAATGCACTTATCCAATATGCTGATTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT230682 WP_000079941.1 NZ_CP090403:c80365-80096 [Shigella sp. PIB]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT230682 NZ_CP125310:4866113-4866409 [Citrobacter freundii]
ATGAAAGACGAATTATTTGCCGATTTGCTGGCCAGTGCAGAAGAAATGGTGCGTATAGAGAAAGGTGAAGAAACACCTAA
GCCTGAACATGTACATACCTTTAGCGAAATTGATGTTAAAGCCATCCGCGAAGCAACAGGGTTACGCCAACAGGATTTTG
CCATAGCTGTTGGCGTGAGTTATGACCTGGTTAAAAGCTGGGAAACGAAACGACGCCAGCCGACTGGTGCACCAAGAAAA
CTGCTTTTATTAATTCAAAAAAATCCATTTATAATTAATCAATTAAAAGCAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5