Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 62298..62823 Replicon plasmid pPIB-1
Accession NZ_CP090403
Organism Shigella sp. PIB

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag LXH19_RS22705 Protein ID WP_001159871.1
Coordinates 62298..62603 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag LXH19_RS22710 Protein ID WP_000813630.1
Coordinates 62605..62823 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXH19_RS22680 (58469) 58469..58885 - 417 WP_001278818.1 plasmid partitioning/stability family protein -
LXH19_RS22685 (58878) 58878..59858 - 981 WP_000688514.1 plasmid segregation protein ParM -
LXH19_RS22690 (60272) 60272..60580 - 309 WP_000030199.1 hypothetical protein -
LXH19_RS22695 (60667) 60667..61311 - 645 WP_001144036.1 ParA family protein -
LXH19_RS22700 (61491) 61491..62297 - 807 WP_000016969.1 site-specific integrase -
LXH19_RS22705 (62298) 62298..62603 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
LXH19_RS22710 (62605) 62605..62823 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LXH19_RS22715 (63381) 63381..64307 + 927 WP_001290416.1 hypothetical protein -
LXH19_RS22720 (64352) 64352..64606 + 255 WP_000678527.1 hypothetical protein -
LXH19_RS22725 (65105) 65105..65449 + 345 WP_000793307.1 hypothetical protein -
LXH19_RS22730 (65625) 65625..66626 - 1002 WP_000774875.1 DUF4238 domain-containing protein -
LXH19_RS22735 (66978) 66978..67232 + 255 WP_000343103.1 hypothetical protein -
LXH19_RS22740 (67232) 67232..67822 + 591 WP_000194550.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCMY-2 - 1..98997 98997


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T230681 WP_001159871.1 NZ_CP090403:c62603-62298 [Shigella sp. PIB]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T230681 NZ_CP125310:c4388326-4387919 [Citrobacter freundii]
GTGGTCCAGTGGCAATCTGATTTACGCGTATCATGGCGCGCGCAGTGGCTTTCACTGCTAATTCATGGGCTGGTCGCGGT
GTTTATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTTTGCTCTCTCTGGTGGTATTCGATAGCG
TTCGCAGTCAGCGTCGAATCAACGCTTGTCAGGGTGAGATTCGGCTGTTGATGGATGGTCGTCTGCGCTGGCAAGGGCAA
GAGTGGTCTATCGTTAGCGCCCCCTGGATGGTTAAAACAGGCATGATGCTGCGTTTGCGCTCTGATGCCGGAAAACGTCA
ACATTTGTGGCTGGCGGCAGACAGTATGGATGATGCAGAGTGGCGGGATTTACGGCGGTTGATGCTGCAAAAGGCGAAGC
AGAAGTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT230681 WP_000813630.1 NZ_CP090403:c62823-62605 [Shigella sp. PIB]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT230681 NZ_CP125310:c4388573-4388307 [Citrobacter freundii]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGCCGCGGTATGCGCGAGCTGGACATCTCGATCATGCCGTT
TTTCGAACATGAATATGACAGCTTAAGTGACGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGATT
TATTTAACTGGTTGATGAATCATGGGAAACCAGCTGATGCTGAGCTGGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References