Detailed information of TA system TA023068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 597067..597707
• Replicon: chromosome
• Accession: NC_011206
• Organism: Acidithiobacillus ferrooxidans ATCC 53993

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LFERR_RS03155
• Protein ID: WP_009567232.1
• Coordinates: 597297..597707 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: B7J4M0
• Locus tag: LFERR_RS03150
• Protein ID: WP_009567231.1
• Coordinates: 597067..597297 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFERR_RS03135	592945..594075	+	1131	WP_012536159.1	threonine synthase	-
LFERR_RS03140	594075..595040	+	966	WP_009567244.1	hypothetical protein	-
LFERR_RS03145	595041..596858	+	1818	WP_012536160.1	single-stranded DNA exonuclease	-
LFERR_RS03150	597067..597297	+	231	WP_009567231.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LFERR_RS03155	597297..597707	+	411	WP_009567232.1	type II toxin-antitoxin system VapC family toxin	Toxin
LFERR_RS03160	597840..598938	+	1099	WP_012606567.1	peptide chain release factor 2	-
LFERR_RS03165	598975..600465	+	1491	WP_009568984.1	lysine--tRNA ligase	-
LFERR_RS03170	600462..601706	+	1245	WP_012536161.1	lipoprotein-releasing ABC transporter permease subunit	-
LFERR_RS03175	601699..602394	+	696	WP_009566877.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-127)

Antitoxin

• SpoVT-AbrB domain (7-43)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15278.46 Da
• Isoelectric Point: 7.3848

>T23068 WP_009567232.1 NC_011206:597297-597707 [Acidithiobacillus ferrooxidans ATCC 53993]
MTLRYLLDTNICIYIAKHHPPAVRERFVQHGADDLAMSVITLGELCWGAEKSQARDKALASIQRLQERIQVAPLTPQVGI
HYGQIRAALESGGRPIGNNDLWIAAHARAEGWIVVTNNEREFRRVEGLQVENWVTA

Nucleotide

• Length: 411 bp

>T23068 NC_011206:597297-597707 [Acidithiobacillus ferrooxidans ATCC 53993]
ATGACCTTGCGTTATCTGCTGGATACCAATATCTGCATTTATATTGCCAAACACCATCCGCCCGCCGTGCGGGAGCGTTT
TGTACAACATGGTGCCGATGATCTGGCGATGTCGGTAATCACCTTGGGTGAATTGTGTTGGGGTGCGGAAAAAAGTCAGG
CGCGTGACAAAGCGTTGGCCAGCATACAGCGCCTGCAAGAACGTATTCAGGTGGCCCCCCTCACCCCTCAGGTCGGCATC
CACTATGGCCAGATCCGCGCGGCTCTGGAAAGCGGCGGGCGGCCCATTGGCAATAATGATCTGTGGATCGCCGCACACGC
CCGCGCCGAAGGCTGGATCGTAGTTACCAACAATGAACGAGAGTTCCGACGGGTGGAAGGATTGCAAGTGGAAAACTGGG
TAACGGCATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8704.76 Da
• Isoelectric Point: 4.4217

>AT23068 WP_009567231.1 NC_011206:597067-597297 [Acidithiobacillus ferrooxidans ATCC 53993]
MAIARIFQSGNSQAVRLPKEFRFEVDQVEIFRRGNEIILREHPVDGTGVFDALADLPEDFMSDGRQDIPPQARENF

Nucleotide

• Length: 231 bp

>AT23068 NC_011206:597067-597297 [Acidithiobacillus ferrooxidans ATCC 53993]
ATGGCCATCGCACGCATCTTCCAATCAGGCAACAGTCAGGCCGTACGGTTGCCCAAAGAATTCCGCTTTGAGGTGGATCA
GGTGGAAATCTTCCGCCGCGGGAATGAGATTATTCTGCGGGAGCATCCAGTGGATGGTACAGGCGTTTTTGATGCCCTGG
CCGACCTTCCCGAAGACTTCATGTCCGACGGACGCCAGGATATTCCACCCCAGGCAAGGGAAAACTTTTGA

Structures

Antitoxin

• AlphaFold DB: B7J4M0