Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4256670..4257167 Replicon chromosome
Accession NZ_CP090402
Organism Shigella sp. PIB

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LXH19_RS20170 Protein ID WP_001173343.1
Coordinates 4256670..4256843 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A2X1N3L1
Locus tag LXH19_RS20175 Protein ID WP_001560287.1
Coordinates 4256871..4257167 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXH19_RS20160 (4253340) 4253340..4254797 + 1458 WP_024045128.1 dipeptide/tripeptide permease DtpC -
LXH19_RS20165 (4255034) 4255034..4256551 + 1518 WP_000003806.1 lysine--tRNA ligase -
LXH19_RS20170 (4256670) 4256670..4256843 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LXH19_RS20175 (4256871) 4256871..4257167 - 297 WP_001560287.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LXH19_RS20180 (4257396) 4257396..4257668 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
LXH19_RS20185 (4257881) 4257881..4261810 - 3930 WP_024046188.1 autotransporter outer membrane beta-barrel domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4256670..4272915 16245


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T230675 WP_001173343.1 NZ_CP090402:c4256843-4256670 [Shigella sp. PIB]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T230675 NZ_CP125310:c1532700-1532482 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11441.94 Da        Isoelectric Point: 4.2924

>AT230675 WP_001560287.1 NZ_CP090402:c4257167-4256871 [Shigella sp. PIB]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMHDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT230675 NZ_CP125310:c1533102-1532728 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A2X1N3L1

References